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- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
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Received October 26, 2022
Revised March 7, 2023
Accepted March 25, 2023
- Acknowledgements
- The authors acknowledge the FEQUI/UFU, CNPq, FAPEMIG (Process: APQ - 00874-18) and CAPES (Brazil) for financial support and also acknowledge the ICP - CSIC institute (Spain) for allowing to this work to be carried out
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Immobilization of commercial acid phosphatases from wheat germ and potato onto ion exchangers
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Abstract
A very simple and fast immobilization technique based on ion exchange was investigated to improve the
thermal stability of acid phosphatase from wheat germ and potato. Immobilization was not efficient for the DEAE-sepharose, and MANAE-agarose supports. On the other hand, Toyopearl DEAE-650s proved to be a promising support,
with immobilization yield above 95% and recovery of activity above 85% for both enzymes. A second step was introduced in the immobilization protocol to improve the thermal stability of these biocatalysts. For this, oxidation and
reduction of glycosidic chains of acid phosphatase were carried out, allowing the formation of aldehyde groups and
subsequent interaction with the amine groups to further stabilize the different forms (free and immobilized). Both biocatalysts showed residual activity after 1 hour of inactivation at the temperature of 60 o
C, a fact not observed for the
free enzyme. The wheat germ acid phosphatase derivative was the most stable, with residual activity of 66.7% for the
only immobilized derivative and 76.2% for the oxidized/reduced derivative. Also, the derivatives prepared by ion
exchange adsorption on Toyopearl (TOYO), followed by oxidation/reduction and intramolecular crosslinking, were
approximately 15 and 41 times more stable than the free enzyme from wheat germ.
Keywords
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