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SEPARATION OF OLIGONUCLEOTIDES BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Korean Journal of Chemical Engineering, October 1989, 6(4), 347-349(3), 10.1007/BF02705224
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Abstract
The use of Reversed-Phase High Performance Liquid Chromatography(RP-HPLC) was investigated for the separation of oligonucleotides. By the gradient elution with the mobile phase of methanol, 0.1M KH2PO4 and 0.02 M MaCl2, the mixtures could be separated in a 250 mm × 4.6 mm I.D. column. The number of theoretical plates and resolution were compared between this technique and Micellar Electrokinetic Capillary Chromatography(MECC). The charged nucleic acid constituents in the RP-HPLC were relatively better separated than in the MECC.
