Print: | ISSN 0304-128X |
Online: | ISSN 2233-9558 |
HWAHAK KONGHAK,
Vol.40, No.4, 488-491, 2002 제조용 재순환 크로마토그래피에 의한 가시오갈피에 포함된 Acanthoside-D의 분리
Separation of Acanthoside-D in Acanthopanax Senticosus by Preparative Recycle Chromatograpy
가시오갈피에 포함되어있는 Acanthoside-D를 추출 및 분리하기 위해서 분석용과 제조용 재순환 크로마토그래피에서의 최적조업조건을 실험적으로 구하였다. 가시오갈피 분말을 에탄올으로 추출하고 헥산으로 분배하였다. 분석용 크로마토그래피에서의 이동상 조건과 주입부피는 각기 water/acetonitrile/methanol=80/14/6 vol%, 20 μl이었고 제조용 재순환 크로마토그래피에서는 각기 70/15/15 vol%, 2 ml이었다. 4번의 재순환을 하여 93%의 순도를 가진 Acanthoside-D를 분취하였다.
To extract and separate Acanthoside-D contained in Acanthopanax Senticosus as a ginseng-like substance, the optimum operating conditions were experimentally determined in the analytical and preparative chromatography. Acanthoside-D was extracted from the powder of the trunk of Acanthopanax Senticosus by enthanol, and the resulting solution was partitioned with n-hexane. The mobile phase composition and injection volume by analytical chromatography were water/acetonitrile/methanol=80/14/6 vol% and 20 μl, respectively while those by recycle chromatography were 70/15/15 vol% and 2 ml. By recycling the sample 4 times, Acanthoside-D with 93% purity was collected.
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