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볏짚 셀룰로오스의 효소가수분해에 의한 글루코오스의 생성 ; -고농도 기질의 가수분해를 중심으로-
Enzymatic Hydrolysis of Rice Straw Cellulose to Glucose ; -In the Range of High Substrate Concentration-
HWAHAK KONGHAK, February 1988, 26(1), 39-44(6), NONE
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Abstract
본 연구는 볏짚에 포함되어 있는 셀룰로오스의 효소가수분해시 반응 제특성과 반응속도식 고찰에 관한 겻으로서 주로 고농도 기질을 대상으로 한 가수분해반응을 연구하였다. 고농도 기질（5-20 w/v%）의 가수분해반응에서 율속단계는 효소와 셀룰로오스의 물리적 흡착단계이고, 반응속도식은 생성물 저해가 있는 변형된 Michaelis-Menten식으로 나타낼 수 있었다.
속도 상수는 ESmax=2.02 unit/㎎ cellulose, Kg=0.0132（unit/ml）-1, k2=0.1628 ㎎ cellulose/
unit·h 그리고 k3=0.1251 ml/㎎ cellulose였다.
0.8 % p-TSA 용액으로 2시간 전처리시킨 분해 볏짚 200 ㎎/ml를 30 unit/ml의 셀룰라아제로서 30시간 반응시켰을 때, 98 %의 글루코오스 전환율을 얻었다.
속도 상수는 ESmax=2.02 unit/㎎ cellulose, Kg=0.0132（unit/ml）-1, k2=0.1628 ㎎ cellulose/
unit·h 그리고 k3=0.1251 ml/㎎ cellulose였다.
0.8 % p-TSA 용액으로 2시간 전처리시킨 분해 볏짚 200 ㎎/ml를 30 unit/ml의 셀룰라아제로서 30시간 반응시켰을 때, 98 %의 글루코오스 전환율을 얻었다.
This study was investigated on the kinetics and characteristics of enzymatic hydrolysis of rice straw cellulose, especially at high substrate concentration for the purpose of effective use of the rice straw.
In the range of high substrate concentration（5-20 w/v%） the rate limiting step of the hydrolysis reaction is physical adsorption step and reaction mechanism was shown as modified Michaelis-Menten equation(product inhibition reaction). The value of kinetioc parameters were ESmax=2,02 unit/mg cellulose, KE=0.0132(unit/ml)-1, k2=0.1628mg cellulose
/unit·h and k3=0.1251 ml/mg cellulose, respectively.
Meanwhile, when 200 mg/ml of milled rice straw（which is pretreated with 0.8% p-TSA for two hours） under 30 unit/ml of cellulase was hydrolyzed for 30 hours, about 98% conversion of glucose was obtained.
In the range of high substrate concentration（5-20 w/v%） the rate limiting step of the hydrolysis reaction is physical adsorption step and reaction mechanism was shown as modified Michaelis-Menten equation(product inhibition reaction). The value of kinetioc parameters were ESmax=2,02 unit/mg cellulose, KE=0.0132(unit/ml)-1, k2=0.1628mg cellulose
/unit·h and k3=0.1251 ml/mg cellulose, respectively.
Meanwhile, when 200 mg/ml of milled rice straw（which is pretreated with 0.8% p-TSA for two hours） under 30 unit/ml of cellulase was hydrolyzed for 30 hours, about 98% conversion of glucose was obtained.
