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초음파 분쇄 리포솜(liposome)의 혼탁도와 안정도
Turbidity and Stability of Sonicated Lecithin Liposomes
HWAHAK KONGHAK, April 1988, 26(2), 171-177(7), NONE
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Abstract
혼탁한 egg yolk phosphatidylcholine의 다층 거대 액포를 cuphorn 시스템으로 초음파 분쇄하여 단층 소액포 리포솜으로 제조하고 그 안정도를 검토하기 위하여 흡광도를 측정하였다. 단층 소액포 수용액은 가시광선 영역에서 Rayleigh 산란이론을 잘 만족하였으며 Smoluchowski의 이론에 따라 빠르게 결집체를 형성한다고 가정하여 흡광도 변화를 예측하였으며, 입자의 크기, 수 그리고 형태가 변해가고 있으며 혼합체의 표면적이 일정하다는 가정으로 실험자료를 설명하였다.
The stability of a small unilamellar liposome, prepared by sonication from the turbid aqueous solutions of egg yolk phosphatidylcholine with the cuphorn ultrasonic system, were investigated by measuring the absorbance of visible light in the range of wavelengths from 425 nm to 750 nm. The absorbance of the freshly sonicated and centrifuged liposome solution followed the linear relationship with 1/wavelength4 of the Rayleigh scattering theory. For the incubated liposomal dispersions, a transient turbidity model was proposed in order to explain the change of turbidity by combining with Smoluchowski fast flocculation, and the model has been shown to be appropriate for the prediction of the changes of liposomal particles, i.e., number, size and shape.