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연속 배양방식에 의한 동물 세포 성장 Model에 관한 연구

Process Analysis of Perfusion System for Mass Cultivation of Animal Cells

HWAHAK KONGHAK, December 1988, 26(6), 659-663(5), NONE
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Abstract

세포 배양기법중 feedback 공법을 변형시킨 연속 배양공정(perfusion chemostat system)에서의 세포 성장 model을 유도했다. 이 model은 기존의 연속 배양방법(continuous culture)과 달리, 세포 성장 속도와 배지 이동속도가 밀접한 관계는 있으나, 같지 않음이 확인됐다. 배양조내의 세포수를 배지의 이동속도에 근거를 둔 성장 model에 의해 간접 측정이 가능했으며, 간접 계산된 최대 세포 성장속도는 0.019(1/h)이었다. 세포의 세포 분리기에의 유착 정도를 알 수 있는 농축농도, f와 recycle비, α가 각각 90.21과 0.01로 측정됐다. 특히 이 측정된 값들은 세포의 대량 배양을 위해 공정의 효율적 조절을 위한 필수적인 수치로 증명됐다.
A mathematical model of Perfusion Chemostat(PC) system is developed by using continuous feedback processes, where dilution rate is no longer equal to specific growth rate. It is found that specific growth rate is strongly related with dilution rate. Mammalian cell density in the bioreactior can indirectly be predicted and estimated by employing growith model as a function of dilution rate. The maximum specific growth rate is 0.019(1/h) at 0.020(1/h) of dilution rate. The recycle ratio, α and concentration factor, f are extimated as 0.01 and 90.21, respectively. These values are proved to be important parameters in controlling PC system to effectively cultivate large quantities of mammalian cells and produce pharmaceutically active proteins.

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