Articles & Issues
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
동물 세포 배양에 의한 물질생산 model 에 관한 연구
Kinetics of Producing Products from Mammalian Cell Cultivation
HWAHAK KONGHAK, June 1989, 27(3), 286-290(5), NONE
Download PDF
Abstract
연속 배양 공법하에서, product 생산을 예측 및 조절할 수 있는 product formation model을 유도해, tPA, EPO와 pro-UK 생산자료에 적용시킨 결과, 세 경우 다 correlation factor가 0.90에서 0.93으로 잘 맞음을 확인했으며, 이 model의 상수인 α와 β가 shear stress 및 용존산소에 크게 영향을 받음을 알 수 있었다. EPO생산을 위한 최적조건은 35%의 용존산소와 25-50rpm이 교반속도였다.
A product formation model including growth associated and maintenance terms is developed for the growth of mammalian cells under Perfusion Chemostat(PC) cultivation. Two parameters in this model, growth asso-ciated term, α, and maintenance factor, β, are estimated for the production of tPA, EPO and pro-UK, and fitting these data with correlation factors of 0.90 to 0.93 for each case. It turned out that essential factors for cell growth, such as shear stress and dissolved oxygen tension play important role with respect to productivity, showing optimal values of 30-40% of dissolved oxygen tension and 20-50rpm of agitation speed for the production of EPO from recombinat CHO cells.
