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고농도 효모 농축법에 의한 삼상탑형 발효조에서의 에탄올 발효

Ethanol Fermentation in a Three Phase Tower Fermenter with Highly Concentrated Yeast Cells

HWAHAK KONGHAK, August 1989, 27(4), 506-512(7), NONE
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Abstract

에탄올 생산성을 높이기 위하여 균체를 두 가지 방법으로 고정화시켜 삼성탑형 발효조에 적용하였다. 고정화 방법으로는 효모 자체 플럭의 빠른 침강에 의한 균체 재순환 농축법과 Ca-alginate구형 겔 내부에 효모를 포괄하여 농축시키는 방법으를 사용하였으며, 혐기 및 준회기 조건에서 에탄올 발효 특성 규명과 이 때의 최적 생산조건을 구하였다.
삼성탑형 발효조에서 균체 재순환 농축법을 이용할 경우 혐기 조건에서 Di(dilution rate)를 0.2hr-1로 하여 200g/l의 글루코오스를 발효시켰을 때 최대 생성 수율인 96%(98g/l)의 에탄올을 얻었으며, 또한 Di 가0.6hr-1일 때 에탄올 최대 생산성은 36g/lㆍhr였다. 한편 DO=0.1ppm 의 준호기 조건에서 고정화 효모 겔을 이용한 에탄올 발효시 Di를 0.2hr-1로 하여 125g/l의 글루코오스를 발효시켰을 때 최대 생성수율인 84.7%(54g/l)의 에탄올을 얻을 수 있었고, Di가 2.0hr-1 일 때 에탄올 최대 생산성은 46g/lㆍhr였다.
Two methods for increasing cell concentration in a fermenter were evaluated in terms of ethanol pro-ductivity; cell recycling with settling of yeast flocs and immobilization of yeast cells in Ca-alginate gels. A three phase tower fermenter was designed and used through this study. Under aerobic and anaerobic conditions, the characteristics of ethanol fermentation process were investigated for each of the methods. It was found that the maximum dry cell concentration in the three phase tower fermenter with floc recycle system was 86g/l. The maximum ethanol yield(96.1%)was obtained at Di=0.2hr-1 under anaerobic condition. The max-imum ethanol productivity in this system was 36g/hrㆍl at Di=0.6hr-1. On the other hand, the fermentation using the Ca-alginate immobilized yeast cell was found to be possible at rel-atively high dilution rates(0.1-0.2hr-1)in the three phase tower fermenter. The maximum ethanol yield in this system was 84.7% at Di=0.2hr-1 under aerobic condition. The maximum ethanol productivity of 46g/hrㆍl was obtained at Di=0.2hr-1.

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