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젤라틴-글루탈알데히드 미소구체의 프로테아제 분해 약물방출 해석
Analysis of Drug Release from Gelatine-Glutaraldehyde Microspheres Accompanying Degradation by Protease
HWAHAK KONGHAK, December 1990, 28(6), 699-705(7), NONE
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Abstract
면실유에 현탁시킨, 카복시플로르세인을 함유한 젤라틴수용액에 글루탈알데히드를 가교결합시켜 10미크론 이하의 미소구체를 제조할 수 있었다.
이 미수구체의 목표지향성 약물치료현상을 37℃, pH 7.4에서 인산염완충액으로 분해효소 존재하에 비생체실험하여 조사하고, 미소구체표면에서 프로테아제분해를 동반하는 확산모델로 그 형광물질의 방출을 해석하였다.
이 모델은 비생체실험에서 의사약물의 방출현상을 잘 모사할 수 있었으며, 이 모델에 의한 확산계수는 0.25×10-9cm2/min이었고, 겉보기 분해속도상수는 0.1358×10-6cm/min이었다.
이 미수구체의 목표지향성 약물치료현상을 37℃, pH 7.4에서 인산염완충액으로 분해효소 존재하에 비생체실험하여 조사하고, 미소구체표면에서 프로테아제분해를 동반하는 확산모델로 그 형광물질의 방출을 해석하였다.
이 모델은 비생체실험에서 의사약물의 방출현상을 잘 모사할 수 있었으며, 이 모델에 의한 확산계수는 0.25×10-9cm2/min이었고, 겉보기 분해속도상수는 0.1358×10-6cm/min이었다.
Microspheres of sizes under 10micron could be obtained by cross-linking gelatine with glutaraldehyde in the suspension of aqueous gelatine solution containing carboxy-fluorescein in cotton seed oil. The site specific medication of the microsphere was investigated in vitro in the existence of degradable enzyme at 37℃, pH7.4 phosphate buffer solution and the release of the fluorescein was analyzed by the diffusion model accom-panying protease degradation at the surface of sphere. The release of the simulated medicine could be described by the diffusion model, and the diffusion coefficient and the rate constant of the enzymatic degradation were 0.25×10-9cm2/min and 0.135×10-6cm/min, respectively.
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