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포도당 산화효소반응을 이용한 3상 실관 생화학반응기의 해석
Analysis of Three-Phase Hollow Fiber Bioreactor Using Enzymatic Glucose Oxidation
HWAHAK KONGHAK, October 1993, 31(5), 547-555(9), NONE
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Abstract
실관의 다공성 부분에 포도당 산화효소를 삽입하고 그 위에 실리콘 코팅을 하여 산소를 통하게 한 모형 삼상실관반응기의 거동에 관한 이론과 실험을 행하였다. 포도당의 농도가 75mM 이상일 때는 산소가 율속단계가 되는 것을 이론과 실험을 통하여 확인하였다. 실험을 통한 유효인자는 예측치 보다 15-25%의 오차속에 잘 일치하였다.
Experimental and theoretical studies on three-phase hollow fiber bioreactor were performed using an asymmetric hollow fiber in which glucose oxidase was entrapped in the porous sponge region and silicone coating was applied on top of that. Oxygen limitation has been confirmed by theory and experiment when the glucose concentration was above 75mM. The measured effectiveness factor was lower by 15-25% than the predicted one, which may be attributed to the deactivation of the enzyme upon immobilization. The theoretical and experimental values were in good agreement within error limits of 15-25%.
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