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Fructosyltransferase와 Glucose Isomerase 혼합효소계를 이용한 프락토 올리고당의 생산

Production of Fructo-oligosaccharides by the Mixed-enzyme System of Fructosyltransferase and Glucose Isomerase

윤종원 노지선 이민규 송승구

Yun JW Noh JS Lee MG Song SK

HWAHAK KONGHAK, December 1993, 31(6), 846-851(6), NONE

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Abstract

프락토 올리고당 생산 효소인 fructosyltransferase와 glucose isomerase와의 혼합효소계를 이용하여 새로운 조성의 프락토 올리고당 생산을 검토하였다. Fructosyltransferase의 효소화학적 특성인 glucose 저해현상을 glu-cose isomerase에 의해 낮추어 주었음에도 불구하고 프락토 올리고당의 전화률을 크게 증가시킬 수 없었다. 이 현상을 설명하기 위한 수학적 모델을 제안하고 실험을 통해 확인한 결과, fructosyltransferase 단일효소계와 비교할 때 혼합효소계의 경우 여러 가지 기질에 대한 동력학적 상수(K_m, K_i)의 값이 현저하게 달라짐을 알 수 있었다. Glucose isomerase에 의해 생산된 fructose 는 fructosyltransferase에 의해 전이되지 않아 프락토 올리고당의 농도를 증가시켜 주지 못하는 것으로 보아 프락토 올리고당의 생산반응을 fructosyltransferase의 selftrans-fer 반응에 의해서만 생산된다는 결론을 얻었다.

The production of fructo-oligosaccharides having new composition was investigated using the mixed-enzyme system of fructosyltransferase and glucose isomerase. Despite of decrease in glucose concentration which acts as an inhibitor of fructosyltransferase during the production of fructo-oligosaccharides, the concentration of fructo-oligosaccharides was not high due to the altered kinetic parameters in the mixed-enzyme system was proposed and compared with the experimental results, which showed good agreement with experimental data. Surpris-ingly, the K_m values and inhibition constant of glucose(K_iG) for fructosyltransferase in the mixed-enzyme system were different from those of fructosyltransferase alone. The fructose isomerized from glucose by glucose isomerase did not act as a sucrose acceptor, which meant the isomerized fructose did not play a part in the production of fructo-oligosaccharides.

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References

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