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고전압장에서 미수계를 이용한 단백질이동
Protein Migration in the High Electrical Field Using the Micro-Aqueous System
HWAHAK KONGHAK, April 1994, 32(2), 206-212(7), NONE
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Abstract
새로이 개발된 전기영동조에서 완충용액 대신 혼합유기용매를 매질로 사용하였고, 단백질용액은 분자수준의 미수계로 제조하여 전극양단에 7KV의 전압을 가하여 단백질의 이동을 시도하였다. 본 연구에서는 유기용매의 전도도를 측정하고 안정된 미수계를 이룰 수 있는 protic 유기용매를 선정하였으며 분자수준 미수계 단백질용액의 용액조성과 온도에 따른 안정성도 조사하였다. 분자수준 미수계의 단백질은 고전압하에서 단백질분자의 미수계 덩이상태로 이동하였고 단백질의 이동방향은 기존의 전기영동과는 반대방향이었으며, 이동속도는 약 100배 이상의 증가가 있다.
Mixed organic solvent was used as a medium instead of buffer solution in the new developed filter paper electrophoresis cell. The sample protein solution was prepared as the micro-aqueous system and the protein of the sample protein solution migrated at the high electrical voltage of 7 kilo volts applied between two electrodes. We could select usable protein organic solvents by measuring the electrical conductivities of organic solvents and checking the stability of the sample protein solution. The protein of the sample protein solution moved lump by lump. The direction of the protein movement was reverse and the migration speed was more than 100 times faster than that of conventional electrophoresis system.
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