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산업용 셀룰라제에 의한 목질계 바이오매스의 동화 특성 연구
Enzymatic Hydrolysis of Lignocellulosic Biomass by Commercial Cellulases
HWAHAK KONGHAK, October 1995, 33(5), 605-609(5), NONE
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Abstract
성분이 다른 산업용 셀룰라제 효소의 목질계 바이오메스에 대한 당화특성을 조사하였다. 셀룰라제 제조 회사에 따라 효소의 구성 성분별 효소 활성이 달랐으며 그 결과 기질과 사용 효소에 따라현저하게 다른 당화능을 보였다. 산업용 셀룰라제 효소 모두 비교적 낮은 β-glucosidase 활성을 가졌으며 그 결과 리그닌이 포함된 기질에서는 β-glucosidase 활성 역제의 효과가 두드러졌다. CMCase 활성이 높을수록 목질계 바이오매스의 효율적인 당화를 기대할 수 있다. FPase 활성 측정은 효소의 리그닌, 헤미셀룰로스 등의 포함된 목질계 바이오매스 기질에 대한 당화능을 적절하게 표현할 수 없는 것으로 나타났다.
Enzymatic hydrolysis of lignocellulosic biomass by various commercial enzymes has been studied. The commercial cellulases used in this study showed different CMCase and β-glucosidase activities but have similar FPase activities. CMCase was found to play a key role for the enzymatic hydrolysis of lignocellulosic biomass which contains inert material such as hemicellulose and lignin. The β-glucosidase inhibition by lignin was the most remarkable for Celluclast which has the lowest β-glucosidase activity. FPase activity could not represent the capability to hydrolyze the lignocellulosic biomass.
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Ado Y, Murata Y, Michiki H, Miyakawa H, Ishibashi H, Ogoshi T, Furuichi M, 에너지이용기술특강, 한국과학기술원, Feb. 5th-7th (1990)
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Brownell HH, Yu EKC, Saddler JN, Biotechnol. Bioeng., 28, 792 (1986)
Goyal A, Ghosh B, Eveleigh D, Bioresour. Technol., 36, 37 (1991)
Persson I, Tjerneld F, Hagerdal H, Bioresour. Technol., 36, 65 (1991)
Breuil C, Chan CM, Gibbert M, Saddler JN, Bioresour. Technol., 39, 139 (1992)
Stockton BC, Mitchell DJ, Groghmann K, Himmel ME, Enzyme Microb. Technol., 13(1), 57 (1991)
Sutcliffe R, Saddler JN, Biotechnol. Bioeng., 17, 749 (1986)
Ghose TK, Pure Appl. Chem., 59(2), 257 (1987)
Miller GL, Anal. Chem., 31, 426 (1959)
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Lowry OH, Resebrough NJ, Farr AL, J. Biol. Chem., 193, 265 (1951)
Duff SJ, Moritz JW, Casavant TE, Biotechnol. Bioeng., 45(3), 239 (1995)
