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산업용 셀룰라제에 의한 목질계 바이오매스의 동화 특성 연구

Enzymatic Hydrolysis of Lignocellulosic Biomass by Commercial Cellulases

HWAHAK KONGHAK, October 1995, 33(5), 605-609(5), NONE
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Abstract

성분이 다른 산업용 셀룰라제 효소의 목질계 바이오메스에 대한 당화특성을 조사하였다. 셀룰라제 제조 회사에 따라 효소의 구성 성분별 효소 활성이 달랐으며 그 결과 기질과 사용 효소에 따라현저하게 다른 당화능을 보였다. 산업용 셀룰라제 효소 모두 비교적 낮은 β-glucosidase 활성을 가졌으며 그 결과 리그닌이 포함된 기질에서는 β-glucosidase 활성 역제의 효과가 두드러졌다. CMCase 활성이 높을수록 목질계 바이오매스의 효율적인 당화를 기대할 수 있다. FPase 활성 측정은 효소의 리그닌, 헤미셀룰로스 등의 포함된 목질계 바이오매스 기질에 대한 당화능을 적절하게 표현할 수 없는 것으로 나타났다.
Enzymatic hydrolysis of lignocellulosic biomass by various commercial enzymes has been studied. The commercial cellulases used in this study showed different CMCase and β-glucosidase activities but have similar FPase activities. CMCase was found to play a key role for the enzymatic hydrolysis of lignocellulosic biomass which contains inert material such as hemicellulose and lignin. The β-glucosidase inhibition by lignin was the most remarkable for Celluclast which has the lowest β-glucosidase activity. FPase activity could not represent the capability to hydrolyze the lignocellulosic biomass.

Keywords

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