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RP-HPLC에서 Deoxyribonucleosides를 분리하기 위한 이동상의 선택도와 구배용매조성법의 최적화

Optimization of Solvent Selectivity and Gradient Mode for Deoxyribonucleosides in RP-HPLC

이주원 노경호

Lee JW Row KH

HWAHAK KONGHAK, October 1997, 35(5), 769-775(7), NONE

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Abstract

RP-HPLC를 이용하여 데옥시리보뉴클레오사이드를 분리하기 위한 최적의 분리조건을 결정하기 위해서 물질의 용량인자와 이동상의 조성에 관한 관계를 이용한 최적화 방법을 사용하였다. 이동상의 조성을 변화시키는 구배용매조성법에서 각 단계에서의 이동상의 조성과 gradient time을 이용한 관계식으로부터 정하였다. 본 연구에서는 이동상으로 물/메탄올,물/아세토나이트릴의 이성분계를 사용하였다. 세 가지 체류모델식에서 용량인자를 이용하여 단 이론으로부터 농도분포곡선을 예측하였다. 계산결과에 의하면 최적의 분리조건은 물/아세토나이트릴로 된 이성분계의 이동상의 조성을 93/7(v/v)에서 5분 동안 유지시키고 이후 5분 동안 91/9로 선형적으로 변화시키는 것이다.

To determine the separation condition for deoxyribonucleosides in RP-HPLC(Reversed-Phase High Performance Liquid Chromatography), the optimization method used was from the relationship of capacity factor and mobile phase composition. In gradient-elution mode by changing mobile phase compositions, the composition and gradient time were determined in stepwise and linear-gradient mode from the relationship. In this work, the binary systems of mobile phase(water/methanol, water/acetonitrile) were utilized. The elution profiles were calculated by the plate theory based on the three retention mechanism of capacity factor. The calculated results showed that in the optimum separation conditions, the mobile phase of water/acetonitrile was maintained for 5 min as 93/7(v/v), then the composition was linearly changed to 91/9 for 5 min.

Keywords

Solvent Selectivity Gradient Mode Optimization Plate Theory Deoxyribonucleosides

References

Snyder LR, Quarry MA, J. Liq. Chromatogr., 10, 1789 (1987)
Kim JD, Row KH, So MS, Polunina IA, Larin AV, J. Liq. Chromatogr., 18, 3091 (1995)
Kim HJ, Hong SI, Korean J. Chem. Eng., 14(5), 382 (1997)
Markowski W, Gotkiewicz W, Chromatohraphia, 25(4), 339 (1988)
Row KH, Larin AV, Korean J. Chem. Eng., 12(4), 442 (1995)
Row KH, Larin AV, Korean J. Chem. Eng., 12(5), 512 (1995)
Choi DC, Choi DK, Row KH, J. Korean Ind. Eng. Chem., 7(5), 985 (1996)
Jung YA, Chung ST, Row KH, HWAHAK KONGHAK, 35(5), 712 (1997)
Chang KK, Row KH, Chung ST, J. Korean Ind. Eng. Chem., 8(2), 286 (1997)
Lee YW, So MS, Lee JW, Chung ST, Row KH, Korean J. Chem. Eng., 13(6), 578 (1996)
Row KH, Larin AV, J. Chem. Eng. Jpn., 28(6), 851 (1995)
Lee JW, Row KH, J. Korean Ind. Eng. Chem., 8(3), 403 (1997)
Lee YW, Row Kh, So MS, Polunina IA, Larin AV, J. Liq. Chromatogr., 18, 3077 (1995)
Polunina IA, Choi DK, Row KH, Larin AV, Colloid J., 58, 805 (1996)