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캡슐 고정화 S. cerevisiae를 이용한 납 회수
Recovery of Lead using Encapsulated S. cerevisiae
HWAHAK KONGHAK, April 1998, 36(2), 229-234(6), NONE
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Abstract
고농도로 미생물을 배양할 수 있는 캡슐고정화법을 이용하여 용액으로부터 분리가 용이하고 재사용 가능한 중금속흡착용 미생물 고정화 캡슐을 개발하였다. 캡슐제조시 효모를 접종하고 36시간 배양하여 건조중량이 250g/L이 되도록 미생물을 캡슐내부에 고정화하였다. 캡슐 고정화 효모의 중금속 흡착 능력은 100ppm의 납용액에서 30mg/g biosorbent이었다. 1M의 염산용액으로 흡착량의 98% 이상을 탈착시킬 수 있었으며 0.1M의 염산용액으로도 85% 이상을 탈착시킬 수 있었다. 캡슐 고정화 효모를 이용한 납 흡착의 최적 pH는 6이었으며 흡착등온식은 기존의 Freundlich 식을 잘 따르고 있었다. 납을 흡탈착하는 공정에 효모 고정화 캡슐을 50회 반복사용한 이후의 흡착용량은 처음과 동일하게 유지되었다.
We prepared a capsule containing Saccharomyces cerevisiae cells for the removal of lead(II) ion. Saccharomyces cerevisiae cells were encapsulated and cultured in the growth medium for 36 hrs. The cells grew in the capsule during cultivation and did not leak through the capsule membrane. The dried cell density reached 250 g/l on the basis of the inner volume of the capsule. The capsule was cross-linked using triethylene tetramine and glutaric dialdehyde solutions. The optimum pH of the lead uptake using encapsulated S. cerevisiae was found to be 6. The Freundlich model showed a little better fit to adsorption data than the Langmuir model. The lead uptake of the encapsulated S. cerevisiae was about 30 mg Pb/g biomass. The 95 percent of leads adsorbed on the encapsulated biosorbents was desorbed by the 1M HCI solution. The capsule was reused 50 batches without losing the metal uptake capacity. And the mechanical strength of the cross-linked capsule was retained after 50 trials.
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