키토산-PVP를 이용하여 유상과 수상의 유효성분을 동시에 함유하는 다중 에멀젼(W/O/W)과 다중 캡슐을 제조하였다. 다중 에멀젼에서 키토산 용액과 PVP를 각각 외부와 내부 수상으로, 그리고 squalane을 내부 유상으로 사용하였다. 다중 에멀젼의 안정성을 증가시키기 위해, 다중 에멀젼을 노즐을 통하여 알칼리용액에서 처리하여 얻은 다중 캡슐은 경화된 벽재(키토산-PVP)로 이루어진 지름 2-3 mm 크기의 비드였다. 키토산과 PVP의 복합막의 경도는 PVP가 증가함에 따라 증가하였다. 항균특성실험에서 E. coli를 사용한 항균특성에서는 1시간이 경과한 후부터 급격한 감소를 보이기 시작하여 24시간에 모두 소멸되는 것으로 나타났다.

Multiple capsule was prepared by using chitosan-PVP multiple emulsion system(W/O/W) in which oil and water phase can co-exist simultaneously. In multiple emulsion, chitosan and PVP solution were used for external and internal aqueous phase materials, respectively. And squalane was used for oil phase material. To increase the stability of multiple emulsion, multiple emulsion was ejected into the NaOH solution through the nozzle to form multiple capsule of 2-3 mm in diameter, where the chitosan-PVP composite was used as a wall materials. The hardness of this multiple capsule was found to increase as the amount of PVP increased. Antibacterial property of this multicapsule was tested by E. coli. After 1 h, there was drastic decrease of E. coli population and all the E. coli was diminished after 24 h.

Multiple Emulsion W/O/W Multiple Capsule Chitosan PVP Antibacterial

Roseman J, Mansdorf SZ, "Controlled Release Delivery Systems," Marcel Dekker, 177 (1983)
Leszek K, "Extended-Release Dosage Form," CRC Press, 114 (1987)
Florence AT, Whitehill D, Int. J. Pharm., 11, 277 (1981)
Beche P, "Emulsion: Theory and Practice," 2nd ed., Reinhold Publishing Corp., New York, 2 (1965)
Brodin AF, Kavaliunas DR, Frank SG, Acta Pharm. Suec., 15, 1 (1978)
Florence AT, Chem. Ind., 20, 1000 (1993)
Chiang C, Fuller GC, Frakenfeld JW, Rhodes CT, J. Pharm. Sci., 67, 63 (1978) 
Li NN, Shrier AL, "Recent Development in Separation Science," CRC Press, Boca Raton, Fla., 163 (1972)
Raghuraman B, Tirmize N, Wiencek J, Environ. Sci. Technol., 28, 1090 (1994) 
Larson K, Raghuraman B, Wiencek J, Ind. Eng. Chem. Res., 33(6), 1612 (1994) 
Li NN, Somerset WJ, U.S. Patent, 3,410,794 (1966)
Lion Dentrific Co. Ltd.: British Patent, 1,541,463 (1979)
Zheng S, Zheng Y, Beissinger RL, Wasan DT, Mccormick DL, Biochim. Biophys. Acta, 65, 1158 (1993)
Kurita K, Koyma Y, Chikaoka S, Polymer, 1083 (1988)
Won JM, Bae SY, Ha BH, Kim HT, Dumazawa H, Korean J. Chem. Eng., 13(3), 324 (1996)
Moon IS, Park SK, Lee SW, Theor. Appl. Chem. Eng., KIChE Spring Meeting (1997)
Fox C, Cosmetic Toiletries, 101 (1986)
Orth DS, Cosmetic Toiletries, 101, 43 (1986)
Klein E, Eichholz E, Theimer F, Yeager D, J. Membr. Sci., 95(2), 199 (1994)