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Trichoderma reesei Rut C-30 유래 cellulase에 의한 고급폐지재생에 관한 연구

Studies on Office-Waste Paper Recycling Using Cellulase from Trichoderma reesei Rut C-30

HWAHAK KONGHAK, October 1998, 36(5), 759-763(5), NONE
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Abstract

본 연구에서는 Trichoderma reesei Rut C-30에서 생산된 crude cellulase를 이용하여 고급폐지 탈묵공정을 수행하였으며 ,상업용 탈묵효소인 Novozym 342를 사용하는 효소적 탈묵공정과 가성소다를 이용하는 화학적 탈묵공정과의 상호 비교실험을 수행하였다. Crude cellulase농도에 따른 탈묵효율은 CMCase activity 기준 2units/g Oven Dry Paper에서 백색도(brightness)와 여수도(freeness)가 최대값을 보였다. 물리적강도의 경우, 여수도와 백색도의 결과와 비슷한 양상을 보였으나 이 농도 이상의 효소를 사용한 경우 물리적 강도가 감소하는 것을 보였다. 그러나 수율은 효소농도 증가에 따라서 지속적으로 감소하는 양상을 보였다. Novozym342또는 가성소다를 사용하는 화학적 탈묵공정과의 비교 실험에서는 Novozym 342에 비해 백색도와 여수도가 우수하였으며, 가성소다를 사용하는 기존의 탈묵공정에 비해서는 탈묵효과 및 물리적 강도가 우수하였다. 그러나 수율면에서는 Novozym 342와 기존의 탈묵공정에 비해서 낮은 결과를 나타냈다. Cellulase에 의한 탈묵공정에서 탈묵효율에 큰 영향을주는 endo성분과 exo성분의 조합비율은 본 실험에서 생산된 crude cellulase의 경우 상업적으로 생산되는 탈묵효소와 비슷한 조합 비율을 보이고 있으며, 이에 근거한 경제성있는 탈묵용 효소로서의 응용을 기대할 수 있을 것으로 생각된다.
Deinking of office-waste paper using crude cellulase from Trichoderma reesei Rut C-30 was studied, and compared with enzymatic deinking with commercial enzyme, Novozym 342, and conventional chemical deinking method using sodium hydroxide. Maximum brightness and freeness were obtained when 2 units of the crude cellulase(CMCase activity unit) was used per gram oven dry paper. Physical strengths of the pulp, deinked with crude cellulase, showed similar behaviors with the brightness and the freeness, but decreased at higher crude cellulase concentration. The recovery yield decreased with the increase of crude cellulase concentration. The brightness and the freeness of deinked pulp using Rut C-30 crude cellulase were better than those using Novozym 342. Enzymatic deinking method gave better results than conventional chemical drinking method in deinking efficiency and physical strengths of deinked pulp. The ratio of endoglucanase and exoglucanase of crude cellulase, known to be an important parameter in determining deinking efficiency and the physical properties, was comparable to that of commercial enzyme.

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