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인 과립구 거식세포 콜로니 자극인자(hGM-CSF) 생산을 위한 효모의 유가배양 연구

Fed-Batch Culture of Yeast for Producting Human Granulocyte Macrophage Colony Stimulation Factor(hGM-CSF)

김인호

Kim IH

HWAHAK KONGHAK, December 1998, 36(6), 972-974(3), NONE

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Abstract

사람의 혈구 조혈 인자의 일종인 인과립구-거식세포 콜로니 자극인자(hGM-CSF)를 분비하는 재조합 효모를 유가배양하였다. 재조합 효모는 알코올 탈수소 효소 프로모터에 의하여 hGM-CSF를 발현하므로 배지 중의 알코올 농도에 따라 hGM-CSF의 발현율이 큰 영향을 받았다. 포도당 공급의 방법에 따라 회분배양, 일정유속의 유가배양, 유속증가의 유가배양을 비교하였다. 이 중 배지 중의 에탄올 농도 변화가 작은 유속증가의 유가배양에서 가장 hGM-CSF발현이 잘 되었고 회분 배양에 비해 단위 OD당 12배의 발현율의 증가가 있었다.

Recombinant yeast strain harboring genes for human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic factor, was cultured in a fed batch mode. Since hGM-CSF is able to be expressed with an aid of alcohol dehydrogenase Ⅱ(ADH Ⅱ)promoter of the yeast strain, ethanol concentration in medium was controlled to obtain a high yield of hGM-CSF. Three culture methods(batch, fed-batch with constant glucose feed, and fed-batch with increasing rate of glucose feed) were compared for the high yield of hGM-CSF. Ethanol concentration was well maintained in the fed batch culture with increasing glucose feed, and consequently yielded a high expression level of hGM-CSF, which was 12 times larger than that of batch culture per unit OD.

Keywords

hGM-CSF Fed Batch Yeast ADHⅡ

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