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Cross-flow 막모듈을 이용한 Cyclodextrins의 효소적 생산과 동시 분리

Enzymatic Production and Simultaneous Separation of Cyclodextrins Using Cross-flow Membrane Module

홍준기 염경호^†

Jun Ki Hong^{1 2} Kyung Ho Youm^1†

충북대학교 공과대학 화학공학부, 청주 361-763

¹School of Chemical Engineering, College of Engineering, Chungbuk National University, Cheongju 361-763, Chungbuk, Korea ²R&D Center, Hanbul Cosmetic Co., Umsung 369-830, Korea

HWAHAK KONGHAK, February 2000, 38(1), 86-91(6), NONE

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Abstract

Cross-flow 막모듈이 설치된 막 생물반응기를 사용하여 가용성 전분으로부터 cyclodextrins(CDs)의 효소적 생산과 동시분리에 대한 연구를 수행하였다. 이 결과 막 반응기는 CDs를 생산 즉시 cross-flow 막모듈을 통해 연속 분리시킴으로써 CDs의 효소 저해 및 선형 maltooligosaccharide로의 전환을 억제시켜 전분의 CDs로의 전환율을 37%로 일정하게 유지시킬 수 있었다. 10%(wt/vol) 전분용액, 2atm의 조작압력 및 60 mL/min의 순환유량 하에서 막 반응기를 24시간 동안 운전하였을 때 CDs의 막 단위 면적당 총 생산량은 6.7kg/㎡ 이었으며, 이때의 α-,β-,γ-CD 각각의 생산비는 약 4.4 : 5.5 : 1 이었다. Cross-flow 막모듈을 설치한 막 반응기에서의 CDs 전체의 생산량과 생산성은 dead-end 막모듈을 설치한 막 반응기의 경우보다 약 1.8-2배 증가하였다.

A study on the enzymatic production and simultaneous separation of cyclodxtrins(CDs) from soluble starch was performed in a membrane bioreactor equipped with the cross-flow membrane module. The conversion of starch to CDs was maintained at a constant value of 37%, since the cross-flow membrane module, in which the separation of CDs from the reaction solution was occurred, suppressed the product inhibition and the breakdown of CDs to the linear maltooligosaccharide. After the reaction for 24 hr using a 10% (wt/vol) solution, the produced amount of total CD per unit membrane area was about 6.7kg/㎡ at the operating pressure of 2 atm and the circulation rate of 600mL/min. Under these operating conditions, the production ratio of α-CD : β-CD : γ-CD was about 4.4 : 5.5 : 1. The produced amount and productivity of CDs in a membrane reactor equipped with the cross-flow membrane module were about 1.8-2 times higher than that in a membrane reactor equipped with the dead-end membrane module.

Keywords

Cyclodextrin Membrane Bioreactor CGTaes Cross-flow Membrane Module

References

Szejtli J, J. Mater. Chem., 7(4), 575 (1997)
Horikoshi K, Process Biochem., May, 26 (1979)
Villiers ACR, Acad. Sci. Paris, 112, 536 (1891)
Schardinger F, Untersuch Z, Nahrungs-Genussmittel Gebrauchsge-genstande, 6, 865 (1903)
Szejtli J, "Cyclodextrin Technology," Kluwer, Dordrecht (1988)
Li S, Purdy WC, Chem. Rev., 92, 1457 (1992)
Lee JE, Choi YS, Kim DI, Korean J. Biotechnol. Bioeng., 12(1), 108 (1997)
Rendleman JA, Biotechnol. Appl. Biochem., 24, 121 (1996)
Vettler D, Thorn W, Brunner H, Konig W, Carbohydr. Res., 223, 61 (1992)
Kim TJ, Lee YD, Kim HS, Biotechnol. Bioeng., 41, 88 (1993)
Corn Products Co.: "Catalogue for Cyclodextrins," 1968 (1968)
Mifune A, Shima J, J. Org. Synth. Jpn., 35, 116 (1977)
Mulder M, "Basic Principles of Membrane Technology," 2nd ed., Kluwer, Dordrecht (1996)
The Membrane Society of Korea: "Membrane Separation: Applications," Jayu Academy, Seoul (1996)
Hong JK, Youm KH, Membrane, 8(3), 170 (1998)
Lejeune A, Sakaguchi K, Imanaka T, Anal. Biochem., 181, 6 (1989)
Kaneko T, Kato T, Nakamura N, Horikoshi K, J. Jpn. Soc. Starch Sci., 34, 45 (1987)
Kato T, Horikoshi K, Anal. Chem., 56, 1738 (1984)
Youm KH, Fane AG, Wiley DE, J. Membr. Sci., 116(2), 229 (1996)
Rendleman JA, Biotechnol. Appl. Biochem., 24, 129 (1996)
Kim TJ, Kim BC, Lee HS, Enzyme Microbial Tech., 20, 506 (1997)