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RP-HPLC에서 완충용액의 농도에 따른 Nucleotides의 체류인자의 예측

Estimation of Retention Factors of Nucleotides by Buffer Concentrations in RP-HPLC

이주원 노경호^†

Ju Weon Lee Kyung Ho Row^†

인하대학교 화학공학과

Dept. of Chem. Eng., Inha Univ., Korea

rowkho@inha.ac.kr

HWAHAK KONGHAK, October 2000, 38(5), 626-632(7), NONE

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Abstract

이동상 내에 있는 buffer의 농도에 따라서 이온성 시료의 체류거동을 예측하기 위하여 RP-HPLC를 이용하여 실험하였다. 사용된 시료는 다섯 가지 nucleotides(5''-CMP, 5''-UMP, 5''-GMP, 5''-IMP, 5''-AMP), buffer로서는 sodium phosphate monobasic과 acetic acid를 사용하였고 농도는 0.01-10 mM까지 변화시켰다. Buffer의 농도와 체류인자에 관한 두 가지 비선형 모델식을 제안하였고 sodium phosphate monobasic과 acetic acid의 농도에 따라서 상관계수는 0.99이상으로 계산 값과 실험 값과 잘 일치하였다.

To estimate the retention behavior of ionized solute by the buffer concentration in mobile phase, the experiments were carried out by RP-HPLC(Reversed-Phase High Performance Liquid Chromatography). Five nucleotides(5''-CMP, 5''-UMP, 5''-GMP, 5''-IMP, 5''-AMP) were used as solutes. Buffers(0.01-10 mM) were sodium phosphate monobasic and acetic acid. We proposed the two nonlinear model equations in terms of buffer concentration to predict the retention factors. It showed the regression coefficients for the concentration of sodium phosphate monobasic and acetic acid were above 0.99, so the calculated by the model equations and experimental value were in good agreements.

Keywords

Nucleotides Retention Factor Buffer Semi-Empirical Equation RP-HPLC

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