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전기장 인가에 따른 DNA 신장에 관한 AFM 연구
An AFM Study for Stretching Electric Field Induced DNA
Jin-Young Park
Sung-Woong Han
Se-Young Son
Seong-Hun Song
Jong-Min Kim1
Toshio Ohtani1
Hiroshi Muramatsu2
Sang-Mok Chang†
동아대학교 공과대학 화학공학과, 부산 604-714 1일본 농림수산청 산하 국립식품연구소, Tsukuba, Ibaraki 305-8642 2일본 세이코 연구소 연구개발센터, Tsukuba, Ibaraki 305-8642
Dept. of Chemical Eng., Dong-A University, Hadan-dong 840, Saha-gu, Busan 604-714, Korea 1Dept. of Food Eng., National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan 2R&D Center, Seiko Instruments Inc., 563 Takatsuka-shinden, Chiba 270-2222, Japan
smjang@mail:donga.ac.kr
HWAHAK KONGHAK, February 2002, 40(1), 100-105(6), NONE
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Abstract
본 연구에서는 마이카 표면상에서 전기장에 의한 극히 낮은 농도의 DNA 신장에 대한 효율적인 방법론을 제시하였다. 전기장에 의한 DNA 신장을 AFM형상으로 분석한 결과, DNA 농도범위가 약 57×10(-3) ng/mL-57×10(-6) ng/mL 일 때 매우 잘 적용되며, 신장된 DNA는 두 전극 중앙에 존재하였다. 또한, 일반 마이카, Mg(2+) 처리된 마이카, 그리고 AP-마이카 표면들에 대한 신장 효율의 차를 기술하였다. 동일한 실험조건 하에서 AP-마이카의 표면이 가장 좋은 효율의 DNA 신장 결과를 나타내었다.
An effective method of DNA stretching using electric field on mica surface was proposed for extremely low solution concentration of DNA. DNA stretching based on electric field was observed by AFM and well applied on the concentration range from 57×10(-3) ng/mL to 57×_x000D_
10(-6) ng/mL. Stretched DNA existed in the middle of two electrodes. The difference in stretching efficiency for the different surfaces of bare mica, Mg(2+) soaked mica and AP-mica was discussed. The best performance of stretching was found from the surface of AP-mica under the same experimental condition.
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Hansma HG, Revenko I, Kim K, Laney DE, Nucl. Acids Res., 24, 713 (1996)
Lyubchenko YL, Jacobs BL, Lindsay SM, Proc. Natl. Acad. Sci. U.S.A., 90, 2137 (1992)
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Kim JM, Chang SM, Muramatsu H, Appl. Phys. Lett., 74, 466 (1999)
