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DNA Chip과 고정화 기술
DNA Chip and Immobilization Technologies
한국과학기술원 생명화학공학과, 대전 305-701 1삼성종합기술원, 대전 305-380
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea 1Samsung Advanced Institute of Technology, Daejeon 305-380, Korea
hgpark@mail.kaist.ac.kr
HWAHAK KONGHAK, June 2002, 40(3), 281-288(8), NONE
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Abstract
DNA chip 기술은, 인간 DNA 염기 서열상의 이상징후와 관련된 질병을 진단하거나 신약 개발을 위하여 특정 DNA 발현의 분석 등에 응용될 수 있다. 현재, DNA chip을 제작하고 DNA hybridization 반응 신호를 감지하는 방법에는 다양한 기술들이 있다. 본 글에서는 급성장하고 있는 DNA 칩의 전반적인 원리, 제조기술, 그리고 DNA를 chip에 고정화 시키는 기술을 중점적으로 소개하고자 한다. 또한 젊은 나이에서의 당뇨 발병과 관련된 유전자인 MODY의 유전자 변이를 진단하는 oligonucleotide DNA chip의 예를 보여주고자 한다.
The application of DNA chip technology includes the diagnosis of various human diseases associated with DNA sequence anomalies and the comprehensive analysis of expressed sequences with regard to newer drug designs. There are a variety of options for making DNA chips and detecting hybridized signals. Here, we take a close look at the principles and_x000D_
manufacturing technologies of this rapidly growing area, highlighting the DNA immobilization technology on the chip. Finally we present an example of oligonucleotide chip for the analysis of multiple MODY gene mutations, which are related with diabetes of the young.
Keywords
References
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Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM, Nature Genet., 21, 10 (1999)
Schena M, Shalon D, heller R, Chai A, Brown P, Davis R, Proc. Natl. Acad. Sci., 93, 10614 (1996)
Guo Z, Guifoyle RA, Thiel AJ, Wang R, Smith LM, Nucleic Acids Res., 22, 5456 (1994)
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Guschin D, Yershov G, Zaslavsky A, Gemmell A, Shick V, Proudnikov D, Arenknov P, Mirzabekov A, Anal. Biochem., 250, 203 (1997)
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Yu CJ, Wan YJ, Yowanto H, Li J, Tao CL, James MD, Tan CL, Blackburn GF, Meade TJ, J. Am. Chem. Soc., 123(45), 11155 (2001)
Taton TA, Mirkin CA, Letsinger RL, Science, 289, 1757 (2000)
Kohler JM, Csaki A, Reichert J, Moller R, Straube W, Fritzsche W, Sens. Actuators B-Chem., 76, 166 (2001)
Derisi J, Penland L, Brown PO, Bittner ML, Meltzer PS, Ray M, Chen T, Su YA, Trent JM, Nature Genetics, 14, 457 (1996)
Lipshutz RJ, Foder SPA, Gingeras TR, Lockhart DJ, Nature Genetics, 21, 20 (1999)
Oyama M, Ikeda T, Lim TK, Ikebukuro K, Masuda Y, Karube I, Biotechnol. Bioeng., 71(3), 217 (2000)
Lab on a chip miniaturation for chemistry and biology, 1, 61 (2001)
Egholm M, Buchardt O, Christensen L, Behrens C, Freier SM, Driver DA, Berg RH, Kim SK, Norden B, Nielsen PE, Nature, 365, 566 (1993)
Kumar R, Singh SK, Koshkin AA, Rajwanshi VK, Meldgaard M, Wengel J, Bioorganic Medicinal Chem. lett., 8, 2219 (1998)
Koshkin AA, Singh SK, Nielsen P, Rajwanshi VK, Kumar R, Meldgaard M, Olsen CE, Wengel J, Tetrahedron, 54, 3607 (1998)
Schena M, Shalon D, Davis RW, Brown PO, Science, 270(5235), 467 (1995)
Shalon D, Smith SJ, Brown PO, Genome Res., 6, 639 (1996)
Cheung VG, Morley M, Aguilar F, Massimi A, Kucherlapati R, Childs G, Nature Genet., 21, 15 (1999)
Okamoto T, Suzuki T, Yamamoto N, Nature Biotechnol., 18, 438 (2000)
Forster AH, Krihak M, Swanson PD, Young TC, Ackley DE, Biosensors Bioelectronics, 16, 187 (2001)
Cuin M, Transfus. Clin. Biol., 8, 291 (2001)
Zammatteo N, Jeanmart L, Hamels S, Courtois S, Louette P, Hevesi L, Remacle J, Anal. Biochem., 280, 143 (2000)
Allemand JF, Bensimon D, Jullien L, Bensimon A, Croguette V, Biophys., 73, 2064 (1997)
Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM, Nature Genet., 21, 10 (1999)
Schena M, Shalon D, heller R, Chai A, Brown P, Davis R, Proc. Natl. Acad. Sci., 93, 10614 (1996)
Guo Z, Guifoyle RA, Thiel AJ, Wang R, Smith LM, Nucleic Acids Res., 22, 5456 (1994)
Froguel P, Velho V, TEM., 10, 142 (1999)
Guschin D, Yershov G, Zaslavsky A, Gemmell A, Shick V, Proudnikov D, Arenknov P, Mirzabekov A, Anal. Biochem., 250, 203 (1997)
Proudnikov D, Timofeev E, Mirzabekov A, Anal. Biochem., 259, 34 (1998)
