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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received August 10, 2004
Accepted January 13, 2005

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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이성분계 단백질 혼합물의 미세막 분리공정에서 막오염에 관한 연구

Fouling Study with Binary Protein Mixtures in Microfilration

안병훈 문동주^† 유계상 ChiaChiHo¹

Byung Hun Ahn Dong Ju Moon^† Kye Sang Yoo Chia Chi Ho¹

한국과학기술연구원 반응매체연구센터, 136-791 서울시 성북구 하월곡동 39-1 ¹신시내티대학교 화공재료공학과, 오하이오주 신시네티시

Reaction Media Research Center, Korea Institute of Science & Technology, 39-1, Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Korea ¹Department of Chemical & Materials Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA

djmoon@kist.re.kr

Korean Chemical Engineering Research, April 2005, 43(2), 236-242(7), NONE Epub 9 May 2005

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Abstract

대표적인 단백질 물질인 bovine serum albumin(BSA), lysozyme, pepsin, casein 그리고 ovalbumin의 이성분계 단백질 혼합물의 미세 분리 막에서 보이는 막 오염 현상에 관한 실험을 고찰하였다. 트랙 에치법에 의해 제조된 0.2 μm 폴리카보네이트 미세 막을 이용하여 단백질 혼합물 용액의 조성과 종류가 여과유량감속에 미치는 영향에 대한 실험을 일정한 압력(14 kPa)과 수소이온농도(pH=11) 하에서 수행하였다. 각각의 이성분 단백질 혼합물에 대한 막오염 현상을 확인하였으며, 유량 감속 데이터는 최근에 개발된 pore blockage-cake formation 모델을 이용하여 분석한 결과, 실험 결과와 일치하였다. 이성분계 단백질 혼합물의 조성과 단백질 종류가 막오염 및 유속변화에 미치는 영향은 공극막힘속도(α), 단백질 침적층의 초기 저항(Rpo) 및 단백질 저항의 증가속도(β)에 의해 설명 할 수 있었다. 대체로 공극막힘속도의 변화는 유속감소현상과 일치함을 알 수 있으며, 특히 casein이 존재하는 단백질 혼합물에서의 급격한 유량감속은 casein에 의한 단백질 침적층의 초기저항에 크게 기인된 것으로 판단된다.

Membrane fouling by protein mixtures during microfiltration has been investigated for binary mixtures of bovine serum albumin (BSA), casein, lysozyme, pepsin, and ovalbumin. Filtration experiments were carried out using 0.2 μm polycarbonate track-etched (PCTE) membrane in a stirred cell under constant transmembrane pressure (14 kPa) and concentration of hydrogen ion (pH=11) to study the effect of mixture composition on filtrate flux decline. Flux decline data were analyzed using a pore blockage-cake formation model developed recently. It was found that the model is in a good agreement with the experimental data. Fouling parameters such as the rate of pore blockage(α), the initial resistance of the protein deposit (Rpo) and the increasing rate of the protein layer resistance(β) were used to evaluate the rate of filtrate flow by membrane fouling in the binary mixture system. Generally, the trend of α is comparable with that of filtrate flux decline. It was also found that fast flux decreasing was observed over the binary mixture containing casein. The result is due to high value of the initial resistance of the protein deposit (Rpo) over casein.

Keywords

Microfiltration Protein Mixtures Blockage-cake Formation Model Fouling Parameters

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