차가버섯(Inonotus obliquus) 균사체의 액체배양에서 온도(22-32 ℃)와 pH(5-7) 그리고 배지성분의 변화가 차가버섯 균사체의 성장에 미치는 연구를 수행하였다. Glucose, starch, peptone, yeast extract, K2HPO4, MgSO4·7H2O 그리고 CaCl2를 각각 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L, 0-0.5 g/L의 범위로 변화시키면서 실험하였다. 차가버섯 균사체의 성장속도는 26-27 ℃, pH 6, glucose 70 g/L, yeast extract 5 g/L 그리고 CaCl2 0.1 g/L일 때에 최대값을 나타났다. Starch와 peptone 그리고 K2HPO4의 농도가 증가할수록 차가버섯 균사체의 성장속도는 증가하였으나, MgSO4·7H2O의 농도가 증가할수록 차가버섯 균사체의 성장속도는 감소하였다. 차가버섯 균사체의 최대 성장 속도를 위한 액체배지는 glucose 70 g/L, peptone 5-20 g/L, starch 10 g/L, yeast extract 5 g/L, K2HPO4 2 g/L 그리고 CaCl2 0.1 g/L로 구성된다.

. Effect of temperature(22-32 ℃), pH(5-7) and medium composition on the mycelial growth for the submerged culture of Inonotus obliquus. The concentrations of glucose, starch, peptone, yeast extract, K2HPO4, MgSO4· 7H2O and CaCl2 were examined in the ranges of 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L and 0-0.5g/L, respectively. The maximum mycelial growth of Inonotus obliquus was obtained for 26-27 ℃ and pH 6. The concentrations of glucose, yeast extract and CaCl2, which gave the maximum mycelial growth of Inonotus obliquus, were 70 g/L, 5 g/L and 0.1 g/L, respectively. In the cases of starch, peptone and K2HPO4, the mycelial growth of Inonotus obliquus increased with increasing the concentrations. However, as the concentration of MgSO4·7H2O increased, the mycelial growth of Inonotus obliquus decreased. The medium for maximum mycelial growth of Inonotus obliquus consisted of (per 1 L): glucose, 70 g; peptone, 5-20 g; starch, 10 g; yeast extract, 5 g; K2HPO4, 2 g and CaCl2, 0.1 g.

Inonotus obliquus Submerged Culture Mycelial Growth Medium Composition

Chang HY, Lee BH, Ko IW, "Inonotus obliqua Strain, Method for Separating It, and Method for Mass Production of Its Fruitbody", Korean Patent No. 10-0339701-0000 (2002)
Hwang YJ, Noh GW, Kim SH, J. Korean Nutr. Soc., 36(1), 18 (2003)
Ham SS, Oh SW, Kim YK, Shin KS, Chang HY, Chung GH, J. Korean. Soc. Food Sci. Nutr., 32(7), 1088 (2003)
Goun EA, Petrichenko VM, Solodnikov SU, Suhinina TV, Kline MA, Cunningham G, Nguyen C, Mile H, J. Ethnopharmacol., 81, 337 (2002) 
Kazuo S, "Liquid Culture of Fuscopia Obliqua (ER.) Aoshima", Japan Patent No. 10,191,783 (1997)
Chung KH, Han JJ, Lee CW , Park JD, Ko EC, "Composition Containing Chaga Mushroom Extract as an Active Ingredient", Korean Patent Unexamined Publication No. 10-2003-0065964 (2003)
Park HJ, Kim YJ, Lee DS, "Manufacture Method of a Functional Tea and Food, Using Extract of Mushroom Mycelium by New Extraction Technique", Korean Patent Unexamined Publication No. 10-2003-0072417 (2003)
Choi KH, Kor. J. Biothechnol. Bioeng., 14(2), 167 (1999)
Kim JM, KIm WJ, "The Chaga Mycelium Culturing Method by Using Unpolished Rice", Korean Patent N. 10-0431327 (2004)
Chung HY, Sung KH, "Method for Cultivating Inonotus Obliqua", Korean Patent Unexamined Publication No. 10-2004-0039936 (2004)
Hong JS, Yun SE, Kim YS, Lee JB, Kor. J. Mycol., 15(2), 108 (1987)
Park KH, Lee JS, Kor. J. Biotechnol. Bioeng., 6(1), 91 (1991)