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Received September 30, 2005
Accepted November 25, 2005
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이온 교환 크로마토그래피에서 온도가 당의 체류 특성에 미치는 영향
Temperature Effect on the Retention Behavior of Sugars in Ion Exchange Chromatography
인하대학교 생물공학과, 402-701 인천시 용현동 253 1인하대학교 초정밀생물분리기술연구센터, 402-701 인천시 용현동 253
Department of Biological Engineering, Inha University, 253, Younghyun-dong, Nam-gu, Incheon 402-751, Korea 1ERC for Advanced Bioseparation Technology, Inha University, 253, Younghyun-dong, Nam-gu, Incheon 402-751, Korea
Korean Chemical Engineering Research, December 2005, 43(6), 722-727(6), NONE Epub 23 January 2006
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Abstract
Dow99Ca350, MFG-220, Finex CS-10GC는 이온 교환 수지로써, 당 분리에 주로 사용되며, 모두 poly styrene DVB를 기본 골격으로 술폰산염, 혹은 술폰산을 작용기로 하고 있다. 이 분리 수지들은 이미 제당 산업의 연속 분리 공정에서 사용되고 있으며, 이에 대해 본 실험은 온도의 영향으로 인한 Dow99Ca350, MFG-220과 Finex CS-10GC의 분리수지에서 당의 체류 혹은 흡착 특성 변화를 알아보기 위하여 수행되었다. 당 분리의 시료로써 사용된 물질은 과당과 포도당으로써 위의 분리 수지와 함께 제당 산업에서 널리 사용된다. Dow99Ca350의 팽윤 시험, 공극률 시험, 펄스 시험, 전단 분석을 통해 온도에 대한 영향을 확인하였다. MFG-220, Finex CS-10GC의 경우, 펄스 시험을 통해 온도 변화에 의한 크로마토그램의 변화를 알아보았다. 실험의 결과 Dow99Ca350, MFG-220과 Finex CS-10GC는 과당과 포_x000D_
도당 각각 최대 1.76, 3.37％의 체류 시간 변화를 보임으로써 온도에 안정적인 경향성을 보였다.
Dow99Ca350 (Dowex monosphere 99Ca/350 separation resin), MFG-220, and Finex CS-10GC are ionexchange resins, and primarily used to separate sugars, and all of these resins have poly styrene DVB backbone, and sulfonyl group. These resins are already used to separate sugars continuously at sugar industry at constant temperature. These resins are used in experiments for understanding temperature effect on retention or adsorption behavior. Using Dow99Ca350, swelling test, porosity test, pulse test, and frontal analysis at various temperatures were performed. In the cases of MFG-220, and Finex CS-10GC, the effect of temperature variation was verified by pulse test. The experimental results are shown that Dow99Ca350, MFG-220, and Finex CS-10GC, which are commercial resins for sugar separation, are stable to temperature variation because the maximum change of retention time of fructose, and glucose are 1.76, and 3.37％ respectively.
Keywords
References
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