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Received May 29, 2006
Accepted June 28, 2006
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미생물을 이용한 절삭유제의 부패성능 평가에 관한 연구
The Study on Decomposition of Metal-working Fluids Against Microbes
한국화학연구원 신화학연구단 유제화학연구센터, 305-600 대전시 유성구 장동 100 1충북전산기계고등학교, 361-823 충북 청주시 홍덕구 분평동 1257
Surfactant & Lubricant Research Team, New Chemistry Division, Korea Research Institute of Chemical Technology, 100, Jang-dong, Yuseong-gu, Daejeon 305-600, Korea 1Chungbuk Mechatronics High School, 1257, Bun-pyeong-dong, Heungduk-gu, Cheong-ju, Chungbook 361-823, Korea
Korean Chemical Engineering Research, August 2006, 44(4), 350-355(6), NONE Epub 6 September 2006
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Abstract
절삭유제의 미생물에 대한 부패능을 평가하기 위하여 미생물의 성장 유형을 조사하고 조절된 균을 절삭유제에 접종하여 부패능을 실험하였다. 미생물의 성장곡선 실험 결과, 균의 대수성장기는 균의 종류에 따라 다르게 나타났으며 E. coli 균의 대수 성장기는 배양 후 2시간, K. pneumoniae 균은 3시간, P. aeruginosa 균은 4시간, P. oleovarans 균은 3시간이었다. OD 값을 0.5로 조절한 접종액의 E. coli 균수는 4.4~10.0×105 CFU/mL, K. pneumoniae 균수는 1.8~9.5×107 CFU/mL이었다. 미생물의 부패에 대한 pH의 영향을 살펴본 결과, pH 6~8에서 부패가 왕성하게 진행되었고 pH가 4 이하, 10이상에서는 균이 성장하지 못함을 알 수 있었다. pH를 중성으로 유지하고 내마모제의 구조에 따른 부패능을 실험한 결과, 에스테르형 내마모제가 에틸렌글리콜형 내마모제 보다 부패가 빠르게 진행되어 구조에 따라 부패능의 차이를 나타내었다.
Growth curves of microbes were examined to evaluate decomposition of metal-working fluids and decomposition properties of metal-working fluids were experimented using controled microbes such as E. coli and K. pnemoniae. According to the results of growth curve of microbes, the growth period depended on species of microbes, 2 h of E. coli, 3 h of K. pneumoniae, 4 h of P. aeruginosa and 3 h of P. oleovarans after incubation. The colony count of E. coli and K. pneumoniae controled to OD of 0.5 ranged from 4.4~10×105 CFU/mL and 1.8×9.5×107 CFU/mL, respectively. The decomposition of metal-working fluids was excellently progressed in the range of pH 6~8 than below pH 4 and above pH 10. In the case of controled fluids to pH 6~8, the decomposition of the fluid containing ester group was more accelerated than that of the fluid containing ethylene glycol.
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Schottenberg AD, “Lubricating Additives,” European Patent No. 183, 050 (1986)
Yong W, Qunji X, Lili C, J. of Synthetic Lubrication, 13(4), 375 (1997)
Canter NM, Chaloupka JJ Fischesser GJ, Lubric. Eng., 44(3), 257 (1987)
Watanabe S, Nakagawa H, Ohmori Y, Tribolosist, 42(1), 81 (1997)
Nakagawa H, Ohmori Y, Watanabe S, Fujita T, Sakamoto M, Tribologist, 43(5), 436 (1997)
CEC-L-33-A-93 Method
OECD 301C Method
MPI, “Cutting Fluids and Grinding Fluids,” pp.228
Yoon YJ, Kim YW, Chugn K, Hwang DH, J. Korean Ind. Eng. Chem., 16(6), 834 (2005)
Shafer WM, “Radical Diffusion Assay,” 175, Section 3.1
Kim MS, “Environmental Microbiology,” Dong-Jong Sa, 97, 170-171
Research Report of Rotus Engineering, “Development of the Decomposition Prevention of Water Soluble Cutting Fluids Using Physical Treatment Method,” (2005)
