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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received January 18, 2006
Accepted June 16, 2006

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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D-tryptophan과 N-CBZ-D-phenylalanine의 분리에서 이온성 액체의 영향

Influence of Ionic Liquid for Separation of D-tryptophan and N-CBZ-D-phenylalanine

김은철 정금주 Yulia Polyakova 구윤모 노경호^†

Yinzhe Jin Jinzhu Zheng Yulia Polyakova Yoon Mo Koo Kyung Ho Row^†

인하대학교 생명화학공학부, 초정밀생물분리기술연구센터, 402-751 인천시 남구 용현동 253

Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University, 253, Yonghyun-dong, Nam-gu, Incheon 402-751, Korea

rowkho@inha.ac.kr

Korean Chemical Engineering Research, October 2006, 44(5), 453-459(7), NONE Epub 14 November 2006

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Abstract

두 개의 D-아미노산 물질을 분리함에 있어서 이온성 액체의 영향을 고찰하였다. 아미노산 물질로서 D-tryptophan과 N-CBZ-D-phenylalanine을 사용하였으며, 이온성 액체로서 1-butyl-3-methylimidazolium tetrafluoroborate([BMIm]+[BF4].)을 사용하였고, 역상 액체 크로마토그래피로 분석하였다. 이동상은 각각 65％, 70％, 80％ 메탄올을 주성분으로 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 10.0, 12.0과 15.0 mmol/L의 농도의 이온성 액체를 첨가하였고, 칼럼(3.9 × 300 mm)에 15 μm의 입자 크기를 가진 C18 실리카 겔을 충진하여 사용하였다. 이온성 액체를 첨가함으로써 D-tryptophan의 체류인자는 거의 변화가 없었지만, N-CBZ- D-phenylalanine의 체류인자는 감소하였다. 두 물질의 분리도는 메탄올의 함량과 이온성 액체의 농도에 따라 변화하였다. 메탄올의 함량이 낮을수록, 이온성 액체의 농도의 증가에 따라서 분리도는 증가하는 경향을 나타내었다.

D-tryptophan and N-CBZ-D-phenylalanine were separated using ionic liquid as additives for the mobile phase in high performance liquid chromatography (HPLC). The ionic liquid of 1-butyl-3- methylimidazolium tetrafluoroborate ([BMIm]+[BF4].) was used. Mobile phases were 65％, 70％, and 80％ methanol in water with addition of different concentrations (0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 10.0, 12.0, and 15.0 mmol/L) of the ionic liquid. The experiments were performed on stainless steel column, 3.9×300 mm i.d., packed with 15 μm octadecyl-bonded silica gel at laboratory. The retention factor of D-tryptophan was not negligibly changed while that of N-CBZ-D-phenylalanine was decreased. The resolution between the two components were affected by the contents of methanol and ionic liquid in the mobile phase. With the small content of methanol and the high concentration of ionic liquid, the resolution was improved.

Keywords

D-tryptophan N-CBZ-D-phenylalanine Ionic Liquid Retention Factor Resolution

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