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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received May 8, 2008
Accepted June 5, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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유류 오염 토양에서 분리된 Rhodococcus fascians를 이용한 JP-8의 분해 특성

Biodegradation of JP-8 by Rhodococcus fascians Isolated from Petroleum Contaminated Soil

남보현 박봉제 윤현식^†

Bo Hyun Nam Bong Je Park Hyun Shik Yun^†

인하대학교 생물공학과, 402-751 인천광역시 남구 용현동 253

Department of Biological Engineering, Inha University, 253 Yong Hyun-dong, Nam-ku, Incheon 402-751, Korea

Korean Chemical Engineering Research, August 2008, 46(4), 819-823(5), NONE Epub 10 September 2008

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Abstract

유류 오염 토양으로부터 항공유 JP-8을 분해하는 능력이 우수한 균주를 분리하여 16S rDNA 염기서열 분석한 결과 Rhodococcus fascians로 확인되었다. 분리된 R. fascians를 MSM 배지에 JP-8을 탄소원으로 배양하여 그 성장특성과 JP-8 분해 특성을 고찰하였다. 분리된 R. fascians는 2% JP-8을 포함하는 배지에서 5일 이내에 JP-8 대부분을 분해하였다. 16% JP-8을 포함하는 배지에서는 10일 동안 배양액 내 JP-8의 농도를 초기 농도의 14%까지 낮출 수 있었다. 17 ℃ 부터 32 oC 까지 JP-8의 분해에 미치는 영향을 살펴본 결과 5일 후에는 모든 시료에서 JP-8의 대부분이 제거되었으며 온도가 낮을수록 R. fascians에 의하여 분해되는 JP-8의 양이 많은 것으로 나타났다. 배지의 초기 pH는 pH5에서 초기 분해속도가 비교적 낮았으나 pH8 까지 모두 5일만에 JP-8의 대부분이 제거되었다.

Microorganisms isolated from petroleum contaminated site were tested for their ability to grow on JP-8 by culturing them on the culture medium that contains JP-8 as a carbon source. The microorganism which grew on JP-8 containing minimal salt medium was separated and identified as Rhodococcus fascians. Changes in JP-8 biodegradation of R. fascians that was isolated from petroleum contaminated site was investigated with various inoculums sizes, JP-8 concentrations, medium pHs, and culture temperatures. The amount of JP-8 was analyzed by TPH using Gas Chromatography.

Keywords

Biodegradation Jet Fuel JP-8 Rhodococcus fascians

References

Margesin R, Walder G, Schinner F, Acta Biotechnol., 1, 29 (2003)
Gallego JLR, Loredo J, Llamas JF, Vazquez F, Sanchez J, Biodegradation, 12, 325 (2001)
Puskas K, Al-Awadhi N, Abdullah F, Literathy P, Environ. Int., 21, 413 (1995)
Rozkov A, Krd A, Vilu R, Biodegradation, 8, 363 (1998)
Mcdougal JN, Pollard DL, Weisman W, Garrett CM, Miller TE, Toxicol. Sci., 55, 247 (2000)
Monteiro-Riviere N, Inman A, Riviere J, J. Appl. Toxicol., 21, 485 (2001)
Riviere JE, Brooks JD, Monteiro-Riviere NA, Budsaba K, Smith CE, Toxicol. Appl. Pharmacol., 160, 60 (1999)
Cookson DJ, Iliopoulos P, Smith BE, Fuel, 74, 70 (1995)
Brink HB, Diane SH, McGraw-Hill, New York, NY (1994)
Boopathy R, Bioresour. Technol., 74(1), 63 (2000)
Block RN, Allworth N, Bishop M, Appl. Microbiol. Biotechnol., 2, 135 (1991)
Eriksson M, Swartling A, Dalhammar G, Appl. Microbiol. Biotechnol., 50(1), 129 (1998)
Marin M, Pedregosa A, Rios S, Ortiz L, Laborda F, Int. Biodeterior. Biodegrad., 35, 269 (1995)
Marquez-Rocha FJ, Hernandez-Rodriguez V, Lamela MT, Water Air Soil Pollut., 128, 313 (2001)
Keith LH, 2nd ed., CRC Press, Inc., New York, NY (1998)