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Received November 18, 2008
Accepted January 9, 2009
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Kromasil HPLC 칼럼에서 온도와 이동상 조성비에 따른 Ketoprofen과 Ibuprofen 라세미체의 분리특성
Effect of Temperature and Eluent Composition on the Separation of Ketoprofen and Ibuprofen Racemates in Kromasil HPLC Column
충남대학교 화학공학과, 305-764 대전시 유성구 궁동 220
Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Korea
ihkim@cnu.ac.kr
Korean Chemical Engineering Research, February 2009, 47(1), 54-58(5), NONE Epub 27 February 2009
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Abstract
Ketoprofen과 Ibuprofen은 비스테로이드 계통의 진통 및 소염제로서 관절염 등을 위한 진통제나 해열제로 이미 널리 사용되고 있다. 그러나 이 두 물질이 치료약으로 사용될 때 (S)-ketoprofen과 (S)-ibuprofen은 약리학적으로 항염증 작용을 하며 (R)-ketoprofen과 (R)-ibuprofen은 약효가 없거나 부작용을 일으키는 문제점을 가지고 있다. 본 연구에서는 Ketoprofen과 Ibuprofen을 Kromasil HPLC 칼럼을 사용하여 이동상 조성비(hexane/t-BME/acetic acid)와 온도 변화 (25~55 ℃)가 분리에 미치는 영향을 실험하였다. 분리특성은 선택도, 분리도, 이론단수 그리고 용량인자와 절대온도의 역수사이의 관계에서 계산된 엔탈피 변화 ΔH에서 Ketoprofen과 Ibuprofen의 kromasil HPLC column 고정상과의 상호_x000D_
작용의 크기를 열역학적으로 검토하였다. 온도 25 ℃, 이동상 조성비(hexane/t-BME/acetic acid) 80/20/0.1에서 선택도, 분리도, 이론단수 엔탈피 수치가 높게 나타났다.
Ketoprofen and ibuprofen are non-steroid anti-inflammatory drug(NSAID) that have analgesic and antipyretic properties. (S)-ketoprofen and (S)-ibuprofen have pharmacological activity, while (R)-ketoprofen and (R)-ibuprofen are either inactive or have side effect. The chiral separation of racemic ketoprofen and ibuprofen enantiomers was carried out by using a Kromasil HPLC column. Some chromatographic parameters (selectivity, resolution, number of theoretical plates and ΔH) are calculated under different mobile phase compositions of hexane/t-BME/acetic acid and temperatures. The selectivity, resolution and number of theoretical plates were observed high at 25 ℃ and the composition of hexane/t-BME/acetic acid (80/20/0.1).
Keywords
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