본 연구에서는 추출용매 물 100%을 이용하여 다양한 초음파 에너지(35, 72, 170 KHz)와 추출 시간(30, 60 min)에의한 고삼으로부터 식물성에스트로겐 제니스테인 및 포르모노네틴의 추출량과 일반성분의 영향을 비교하였다. 전처리단계는 초음파추출, 여과, 농축, 막분리로 구성되었다. 추출된 용액은 역상 고성능 액체크로마토그래피 (HPLC)를 사용하여 분석하였다. 이동상 조성은 A는 물/아세트산(99.9/0.1 vol%), B는 아세토니트릴/아세트산(99.9/0.1 vol%)이며, A/B를 80/20~65/35 vol%로 60분 동안 선형적으로 변화시켰다. 실험결과에 의하면 일반성분은 탄수화물(0.255~0.413%)을 제외한 나머지 성분들의 함량은 거의 비슷하게 확인되었다. 또한 주파수 170 KHz, 60 min에서 추출량이 3.17 g으로 추출 수율이 가장 우수하였고 천연물관련 화학 및 생물학 연구에 기초자료로 도움이 될 것이다.

In this work, we the ettect on extraction amounts and general composition content of phytoestrogen genistein and formononetin extracted from Sophoraflavescens Aiton by various ultrasonic waves(35, 72, and 170 KHz) and extraction time(30, and 60 min) were compared using extraction solvent water 100%. The pretreatment step was composed of ultrasonic waves extraction, filtration, concentration, and membrane filtration. The extracted sample was analyzed by reversed-phase high performance liquid chromatography(RP-HPLC). And the mobile phase applied was linearly changed with A/B of 80/20~65/35 vol% for 60 min(A water/acetic acid, 99.9/0.1 vol%, B acetonitrile/acetic acid, 99.9/0.1 vol%). The experimental results, general composition carbohydrate(0.255 to 0.413%) excepts, other ingredients was confirmed almost similarly. Also, The highest yield of extraction amount 3.17g was obtained by ultrasonic waves with a frequency of 170 KHz and an extraction time of 60 min. This work offers would be useful for chemical and biological studies of natural plants and its products.

Extraction Purification Phytoestrogen Sophoraflavescens Aiton Ultrasound

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