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Received July 8, 2009
Accepted August 3, 2009
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원자간력 현미경을 이용한 단일세포 조작 및 고효율 유전자 도입기술
Atomic Force Microscopy(AFM) based Single Cell Manipulation and High Efficient Gene Delivery Technology
일본이화학연구소 세포시뮬레이션팀 1일본산업종합기술연구소 세포엔지니어링연구부문 2경희대학교 화학공학과, 446-701 경기도 용인시 기흥구 서천동 1번지 3동아대학교 화학공학과, 604-714 부산시 사하구 하단 2동 840
Computational Cell Biomechanics Team, VCAD System Research Program, Riken Headquarters, 2-1, Hirosawa, Wako-shi, Saitama, 351-0198, Japan 1Research Institute for Cell Engineering, AIST, Central 6 1-1-1 Higashi, Tsukuba, Ibaraki 105-8566, Japan 2Department of Chemical Engineering, Kyung Hee University, 1 Seocheon-ri, Kiheung-up, Yongin, Gyeonggi 446-701, Korea 3Department of Chemical Engineering, Dong A University, 840 Hadan2-dong, Saha-gu, Busan 604-714, Korea
smjang@dau.ac.kr
Korean Chemical Engineering Research, October 2009, 47(5), 538-545(8), NONE Epub 5 November 2009
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Abstract
본 총설에서는 주사형프로브현미경의 원리와 응용에 관하여 간략히 설명하고 최근 본 그룹에 의하여 활발하게 연구되고 있는 나노탐침과 AFM(원자간력현미경 atomic force microscopy)을 이용한 저침습성(low-invasive) 단일세포 조작기술과 고효율 유전자 도입기술을 소개하고자 한다. 시판 AFM 탐침을 침상구조로 가공한 나노탐침과 AFM을 이용하였을 경우, 탐침의 세포삽입의 성공여부를 force-distance curve 상의 척력소실의 유무로 판단 할 수 있다. 침상 나노탐침을 사용하면 대부분의 세포에서 80~90%의 고효율 세포삽입이 가능하여 마이크로인젝션용 미세관을 이용하는 경우보다 세포삽입효율이 높았다. 또한 나노탐침의 직경이 400 nm 이하의 경우에는 세포 종류에 관계없이 장시간 나노탐침의 삽입에도 세포활성에 큰 영향이 없었다. 침상나노탐침을 이용하여 DNA를 도입하였을 경우에도 기존의 DNA 도입방법과 비교하여 높은 도입효율과 유전자 발현율로 DNA를 도입할 수 있는 가능성을 확인하였다.
The principle and application of a scanning probe microscopy(SPM) are reviewed briefly, and a low-invasive single cell manipulation and a gene delivery technique using an etched atomic force microscopy(AFM) probe tip, which we call a nanoneedle, are explained in detail. The nanoneedle insertion into a cell can be judged by a sudden drop of force in a force-distance curve. The probabilities of nanoneedle insertion into cells were 80~90%, which were higher than those of typical microinjection capillaries. When the diameter of the nanoneedle was smaller than 400 nm, the nanoneedle insertion into a cell over 1 hour had almost no influence on the cell viability. A highly efficient gene delivery and a high ratio of expressed gene per delivered DNA compared the conventional major nonviral gene delivery methods could be achieved using the gene modified nanoneedle.
References
Chang SM, Kim JM, Chem. World., 36, 21 (1996)
Chang SM, Lee HJ, Kim JM, Hiroshi M, Prospect. Ind. Chem., 2(4), 52 (1999)
Takahashi K, Yamanaka S, Cell, 126, 663 (2006)
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S, Cell, 131, 1 (2007)
Hahn W Ho SH, Jeong JG, Hahn EY, Kim S, Yu SS, Kim S, Kim JM, Gene Ther., 11, 739 (2004)
Selkirk SM, Green SJ, Plunkett RJ, Baron TA, Lis A, Spence PO, Gene Ther., 9, 432 (2002)
Kang Y, Stein CS, Heth JA, Sinn PL, Penisten AK, Staber PD, Ratliff KL, Shen H, Barker CK, Martins I, Sharkey CM, Sanders DA, McCray PB, Davidson BL, J. Virol., 76, 9378 (2002)
Modlich U, Bohne J, Schmidt M, von Kalle C, Knoss S, Schambach A, Baum C, Blood, 108, 2545 (2006)
Mathis JM, Williams BJ, Sibley DA, Carroll JL, Li J, Odaka Y, Barlow S, Nathan CO, Li BD, DeBenedetti A., J. Gene Med., 8, 1105 (2006)
Bruder JT, Kovesdi I, J. Virol., 71, 398 (1997)
Shifrin AL, Chirmule N, Gao GP, Wilson JM, Raper SE, Pancreas, 30, 122 (2005)
Bieber T, Meissner W, Kostin S, Niemann A, Elsasser HP, J. Control. Release, 82, 441 (2002)
Hoelters J, Ciccarella M, Drechsel M, Geissler C, Gulkan H, Bocker W, Schieker M, Jochum M, Neth P, J. Gene Med., 7, 718 (2005)
Jeschke MG, Barrow RE, Hawkins HK, Yang K, Hayes RL, Lichtenbelt BJ, Perez-Polo JR, Herndon DN, Gene Ther., 6, 1015 (1999)
Pollard H, Remy JS, Loussouarn G, Demolombe S, Behr JP, Escande D, J. Biol. Chem., 273, 7507 (1998)
Vijayanathan V, Thomas T, Thomas TJ, Biochemistry, 41, 14085 (2002)
Knoblauch M, Hibberd JM, Gray JC, van Bell AJ, Nat. Biotechnol., 17, 906 (1999)
Tsulaia TV, Prokopishyn NL, Yao A, Carsrud NDV, Carou MC, Brown DB, Davis BR, Yannariello-Brown J, J. Biomed. Sci., 10, 328 (2003)
Han S, Nakamura C, Imai Y, Nakamura N, Miyake J, Biosens. Bioelectron., 24, 1219 (2008)
Han S, Nakamura C, Kotobuki N, Obataya I, Ohgushi H, Nagamune T, Miyake J, Nanomedicine, 4, 215 (2008)
Han S, Nakamura C, Obataya I, Nakamura N, Miyake J, Biosens. Bioelectron., 20, 2120 (2005)
Han SW, Nakamura C, Obataya I, Nakamura N, Miyake J, Biochem. Biophys. Res. Commun., 332(3), 633 (2005)
Nakamura C, Kamiishi H, Nakamura N, Miyake J, Electrochemistry, 76, 586 (2008)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Nano Lett., 5, 27 (2005)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Biosens. Bioelectron., 20, 1652 (2005)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Nanobiotechnology, 1, 347 (2005)
Mathur AB, Truskey GA, Reichert WM, Crit. Rev. Biomed. Eng., 28, 197 (2000)
Rotsch C, Jacobson K, Radmacher M, Proc. Natl. Acad. Sci., 96, 921 (1999)
Boron WF, Adv. Physiol. Educ., 28, 160 (2004)
Doi A, Mizuno M, Katafuchi T, Furue H, Koga K, Yoshimura M, Eur. J. Neurosci., 26, 2565 (2007)
Chang SM, Lee HJ, Kim JM, Hiroshi M, Prospect. Ind. Chem., 2(4), 52 (1999)
Takahashi K, Yamanaka S, Cell, 126, 663 (2006)
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S, Cell, 131, 1 (2007)
Hahn W Ho SH, Jeong JG, Hahn EY, Kim S, Yu SS, Kim S, Kim JM, Gene Ther., 11, 739 (2004)
Selkirk SM, Green SJ, Plunkett RJ, Baron TA, Lis A, Spence PO, Gene Ther., 9, 432 (2002)
Kang Y, Stein CS, Heth JA, Sinn PL, Penisten AK, Staber PD, Ratliff KL, Shen H, Barker CK, Martins I, Sharkey CM, Sanders DA, McCray PB, Davidson BL, J. Virol., 76, 9378 (2002)
Modlich U, Bohne J, Schmidt M, von Kalle C, Knoss S, Schambach A, Baum C, Blood, 108, 2545 (2006)
Mathis JM, Williams BJ, Sibley DA, Carroll JL, Li J, Odaka Y, Barlow S, Nathan CO, Li BD, DeBenedetti A., J. Gene Med., 8, 1105 (2006)
Bruder JT, Kovesdi I, J. Virol., 71, 398 (1997)
Shifrin AL, Chirmule N, Gao GP, Wilson JM, Raper SE, Pancreas, 30, 122 (2005)
Bieber T, Meissner W, Kostin S, Niemann A, Elsasser HP, J. Control. Release, 82, 441 (2002)
Hoelters J, Ciccarella M, Drechsel M, Geissler C, Gulkan H, Bocker W, Schieker M, Jochum M, Neth P, J. Gene Med., 7, 718 (2005)
Jeschke MG, Barrow RE, Hawkins HK, Yang K, Hayes RL, Lichtenbelt BJ, Perez-Polo JR, Herndon DN, Gene Ther., 6, 1015 (1999)
Pollard H, Remy JS, Loussouarn G, Demolombe S, Behr JP, Escande D, J. Biol. Chem., 273, 7507 (1998)
Vijayanathan V, Thomas T, Thomas TJ, Biochemistry, 41, 14085 (2002)
Knoblauch M, Hibberd JM, Gray JC, van Bell AJ, Nat. Biotechnol., 17, 906 (1999)
Tsulaia TV, Prokopishyn NL, Yao A, Carsrud NDV, Carou MC, Brown DB, Davis BR, Yannariello-Brown J, J. Biomed. Sci., 10, 328 (2003)
Han S, Nakamura C, Imai Y, Nakamura N, Miyake J, Biosens. Bioelectron., 24, 1219 (2008)
Han S, Nakamura C, Kotobuki N, Obataya I, Ohgushi H, Nagamune T, Miyake J, Nanomedicine, 4, 215 (2008)
Han S, Nakamura C, Obataya I, Nakamura N, Miyake J, Biosens. Bioelectron., 20, 2120 (2005)
Han SW, Nakamura C, Obataya I, Nakamura N, Miyake J, Biochem. Biophys. Res. Commun., 332(3), 633 (2005)
Nakamura C, Kamiishi H, Nakamura N, Miyake J, Electrochemistry, 76, 586 (2008)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Nano Lett., 5, 27 (2005)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Biosens. Bioelectron., 20, 1652 (2005)
Obataya I, Nakamura C, Han S, Nakamura N, Miyake J, Nanobiotechnology, 1, 347 (2005)
Mathur AB, Truskey GA, Reichert WM, Crit. Rev. Biomed. Eng., 28, 197 (2000)
Rotsch C, Jacobson K, Radmacher M, Proc. Natl. Acad. Sci., 96, 921 (1999)
Boron WF, Adv. Physiol. Educ., 28, 160 (2004)
Doi A, Mizuno M, Katafuchi T, Furue H, Koga K, Yoshimura M, Eur. J. Neurosci., 26, 2565 (2007)
