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Received June 8, 2009
Accepted August 18, 2009
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초임계 이산화탄소에서 밀배아유의 효소적 에탄올화 반응 특성
Characteristics of Wheat Germ Oil during Enzymatic Ethanolysis in Supercritical Carbon Dioxide
Sung-Sin Back
Kyung-Tae Kwon
Go-Woon Jung
Hyaung-Min Ahn
Jeong-Eun Sim
Hee-Moon Kang
Byung-Soo Chun†
부경대학교 식품공학과, 608-737 부산시 남구 대연 3동 595-1
Faculty of Food Science and Biotechnology, Pukyong National University, 595-1 Deayon-3 dong, Nam-gu, Busan 608-737, Korea
bschun@pknu.ac.kr
Korean Chemical Engineering Research, October 2009, 47(5), 546-552(7), NONE Epub 5 November 2009
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Abstract
이번 연구에서는 밀배아유의 기능성 향상을 위해 고정화 효소를 이용한 밀배아유의 효소적 에탄올 반응을 수행했고, 효소적 에탄올 반응의 비가압조건과 가압조건을 중점적으로 비교·분석했다. 비가압조건 효소적 에탄올 반응 수행은 밀배아유와 99.9% 에탄올 혼합물에 두 가지 고정화 효소인 Lipozyme TL-IM과 Lipozyme RM-IM를 1~5 w%(밀배아 기준 무게비)로 25 ml 플라스크에 shaking machine 상에서 40~70 ℃, 120 rpm 조건으로 실험을 수행했다. 가압조건상에서의 효소적 에탄올 반응 조건은 고정화 효소 2 w%, 반응 시간 24시간, 반응 온도 40~60 ℃ 및 반응 압력 75, 100, 150, 200 bar으로 수행했다. 실험으로부터 회수된 sample은 트리글리세라이드의 분해 정도를 살펴보기 위해 모노-, 디-, 트리글리세라이드를 HPLC를 이용하여 분석했다. 밀배아유의 전반적인 전환율은 반응온도와 고정화 효소의 농도에 따라 증가했고, 최적 반응 조건은 가압조건 50 ℃, 100 bar이었다.
Enzymatic ethanolysis of wheat germ oil with immobilized lipase was investigated for enhancing the function of wheat germ oil. Ethanolysis reactions were carried out in two different systems; non-pressurized and pressurized system. In non-pressurized system, the enzymatic ethanolysis was carried out in an erlenmeyer flask(25 ml) containing a mixture of wheat germ oil and 99.90% ethanol using 1~5 wt% immobilized lipase as Lipozyme TL-IM and Lipozyme_x000D_
RM-IM and the reaction mixtures were incubated at 40~70 ℃ with 120 rpm shaking. In pressurized system, the enzymatic ethanolysis was carried out at various condition; immobilized lipase concentration(2 wt%), reaction time(24 h), reaction temperature(40~60 ℃) and reaction pressure(75, 100, 150, 200 bars). The samples obtained from each fraction were analyzed by HPLC for analysing contents of monoglyceride, diglyceride, and triglyceride. The conversion of_x000D_
wheat germ oil relied on the reaction temperature and the concentration of immobilized lipase. The optimum condition of enzymatic ethanolysis in non-pressurized and pressurized systems was at 50 ℃ and 100 bar.
Keywords
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