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Received October 30, 2009
Accepted December 10, 2009
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바이오디젤 생산을 위한 리파아제 고정 부직포의 효소활성화
Enzyme Activity of Lipase Immobilized Non-Woven Fabric for Biodiesel Production
동국대학교 안전환경시스템공학과, 780-714 경상북도 경주시 석장동 707 1동국대학교 화공생물공학과, 100-715 서울시 중구 필동 3가 26번지
Department of Safety Environmental System Engineering, Dongguk University, 707 Seokjang-dong, Gyeongju-si, Gyeongbok 780-714, Korea 1Department of Chemical and Biochemical Engineering, Dongguk University, , 26 Pil-dong, 3-ga, Jung-gu, Seoul 100-715, Korea
sjpark@dongguk.edu
Korean Chemical Engineering Research, February 2010, 48(1), 121-127(7), NONE Epub 4 March 2010
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Abstract
본 연구의 목적은 효소법을 이용한 바이오디젤의 생산에서의 효소(lipase) 활성화를 최적화함에 있다. 효과적인 방법으로 효소를 고정하기 위해 방사선 그라프트 중합법을 이용한 부직포에 음이온 교환기인 ethanolamine과 diethylamine을 도입시켜 음이온 교환 부직포(이때 얻어진 부직포는 EtA, DEA-EtA 부직포라 함)를 합성하였다. 기존에 사용하던 다공성 중공사막의 경우 시간이 지남에 따라 막힘 현상에 따라 유속이 현저하게 줄어드는 점을 보완하고자 기공(pore size)이 300 μm인 부직포를 선택하였다. 이 부직포에 음이온 교환기가 도입된 EtA, DEA-EtA 부직포의 최적효소 흡착 특성과 효소 활성도에 대하여 고찰하였다. 그 결과 효소 흡착량은 EtA, DEA-EtA 부직포가 비슷하였으나(EtA nonwoven fabric: 15.69 mg/g, DEA-EtA non-woven fabric: 14.45 mg/g) 기름을 투과시킨 결과 효소 활성화는 DEA-EtA_x000D_
부직포가 EtA 부직포에 비해 현저히 떨어짐(EtA non-woven fabric: 3.50mol/h·kg, DEA-EtA non-woven fabric: 0.38mol/h·kg)을 알 수 있었다. 이 음이온교환기를 이용해 효율적인 바이오디젤 생산을 위한 온도, 효소고정량, 기름과 알코올과의 관계 등의 최적의 조건을 도출하였다.
This study is to optimize the enzyme(lipase) activity for biodiesel production. The ion-exchanged nonwoven fabrics(EtA, DEA-EtA non-woven fabric) containing ethanolamine, diethylamine groups are used by radiation induced grafted polymerization onto a non-woven fabric for more effective immobilization of lipase. Since the porous hollow fiber membranes are showed the low throughputibehe non-woven fabric membranes are used for biodiesel production. The physical charateristics of enzyme immobilized and the enzyme activity to EtA and DEA-EtA non-woven_x000D_
fabrics are studied. The EtA non-woven fabrics are quite similar to DEA-EtA non-woven fabric for the amount of enzyme immobilized(EtA non-woven fabric:15.69 mg/g, DEA-EtA non-woven fabric:14.45 mg/g) but DEA-EtA nonwoven fabrics have shown the lower permeabiliquite the organic solvent than the EtA non-woven fabrics(EtA non-woven fabric:3.50 mol/h·kg, DEA-EtA non-woven fabric:0.38 mol/h·kg). Optimum characteristics of ehe non-woven fabric membranes and the limilaractivity are also investigated for the effective biodiesel production.
Keywords
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