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Received November 4, 2009
Accepted November 28, 2009
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생체적합성 고분자를 사용한 다층 조립 구조 캡슐의 제조와 특성
Preparation and Characterization of Multilayer Microcapsules using Biocompatible Polymers
1부산대학교 고분자공학과, 609-735 부산시 금정구 장전동 산 30 2한국의류시험연구원 부산지원, 612-020 부산시 해운대구 우동 1470 3동서대학교 에너지/생명공학부, 617-716 부산광역시 사상구 주례2동 산 69-1번지
1Department of Polymer Science and Engineering, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan 609-735, Korea 2Korea Apparel Testing and Research Institute, 1470 U-dong, Haeundae-gu, Busan 612-020, Korea 3Energy and Bio Engineering, Dongseo University, San 69-1, Churye-2-dong, Sasang-gu, Busan 617-716, Korea
csha@pnu.edu
Korean Chemical Engineering Research, April 2010, 48(2), 178-184(7), NONE Epub 3 May 2010
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Abstract
본 논문에서는 생체적합성 고분자들의 자기 조립 특성을 이용하여 마이크론 단위의 캡슐을 제조하여, 캡슐 내부에 단백질을 넣고 시간과 pH에 따른 방출 거동을 고찰하였다. 본 연구에서는 키토산과 헤파린 그리고 알지네이트를 사용하여 동공(hollow) 캡슐을 제조하였다. 멜라민과 포름알데히드를 일정 비율로 혼합하여, 표면에 전하를 가지는 마이크론 단위의 core를 제조한 후, 음전하를 가지는 헤파린 혹은 알지네이트를 core 위에 흡착시키고, 양전하를 띠는 키토산을 흡착시킨 후, core 위에 교대로 흡착시켜 Multilayer를 형성시켰다. 4층을 쌓은 후에 HCl을 이용하여 pH 2로 조절하면, core는 제거되고 속이 비어 있는 캡슐을 제조할 수 있었다. 동공 캡슐은 투과전자현미경, 표면주사현미경 및 광학 현미경으로 관찰하였다. 이러한 캡슐은 pH에 따라서 각기 다른 거동을 보이는데, 본 연구에서는 내부에 FITC-albumin을 넣어 UV분광기로 방출되는 상대적인 양을 관찰한 결과, 키토산-헤파린 캡슐과 키토산-알지네이트 캡슐은 각기 다른 pH에서 개폐됨을 알 수 있었다.
The aim of this work is the fabrication of polyelectrolyte microcapsules composed of biocompatible polymers such as chitosan, heparin and alginate, to encapsulate the fluorescein isothiocyanate(FITC)-albumin, and to investigate the protein release behavior therefrom. Polyelectrolyte capsules with 4-layer structures could be prepared with biocompatible materials by oppositely charged adsorption using melamin-foramide as a template. Transmission electron_x000D_
microscope(TEM), scanning electron microscope(SEM) and optical microscope confirmed hollow capsule structures. Protein release before and after encapsulation was monitored with a UV-Vis spectrometer. Microcapsules have different behaviors depending on the kind of polyelectrolyte polymers, chitosan-heparin capsules or chitosan-alginate capsules. In conclusion, the polyelectrolyte multilayer shells can be switched between an open and closed state by means of tuning the pH value.
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Caruso F, Caruso RA, Mohwald H, Science, 282(5391), 1111 (1998)
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Lvov Y, Antipov AA, Mamedov A, Mohwald H, Sukhorukov GB, Nano Lett, 1, 125 (2001)
Sukhorukov GB, Donath E, Lichtenfeld H, Knippel E, Knippel M, Budde A, Mhwald H, Coll. Surf. A, 137, 253 (1998)
Sukhorukov GB, Brumen M, Donath E, Mohwald H, J. Phys. Chem. B, 103(31), 6434 (1999)
Sukhorukov GB, Donath E, Moya S, Susha AS, Voigt A, Hartmann J, Mohwald H, J. Microencapsul., 17(2), 177 (2000)
Donath E, Sukhorukov GB, Caruso F, Davis SA, Mhwald H, Angew. Chem. Int. Ed, 37, 2201 (1998)
Moya S, Sukhorukov GB, Auch M, Donath E, Mohwald H, J. Colloid Interface Sci., 216(2), 297 (1999)
Voigt A, Lichtenfeld H, Sukhorukov GB, Zastrow H, Donath E, Baumler H, Mohwald H, Ind. Eng. Chem. Res., 38(10), 4037 (1999)
Sukhorukov G, Dahne L, Hartmann J, Donath E, Mohwald H, Adv. Mater., 12(2), 112 (2000)
Sukhorukov GB, Mobius D, Miller R (Eds.), Elsevier, Amsterdam, 384 (2001)
Radtchenko IL, Giersig M, Sukhorukov GB, Langmuir, 18(21), 8204 (2002)
Sukhorukov GB, Antipov AA, Voigt A, Donath E, Mohwald H, Macromol. Rapid Commun., 22(1), 44 (2001)
Tiourina OP, Sukhorukov GB, Int’l. J. Pharm., 242, 155 (2002)
Kabanov V, Zezin A, Pure Appl. Chem, 56, 343 (1984)
Philipp B, Dautzenberg H, Linow KJ, Kotz J, Dawydof W, Prog. Polym. Sci, 14, 91 (1989)
Decher G, Science, 277(5330), 1232 (1997)
Antipov A, Sukhorukov GB, Leporatti S, Radchenko I, Donath E, Moehwald H, Coll. Surf., A, 198, 535 (2002)
Caruso F, Caruso RA, Mohwald H, Science, 282(5391), 1111 (1998)
Qiu XP, Leporatti S, Donath E, Mohwald H, Langmuir, 17(17), 5375 (2001)
Lvov Y, Ariga K, Ichinose I, Kunitake T, J. Am. Chem. Soc., 117(22), 6117 (1995)
Fang M, Grant PS, McShane MJ, Sukhorukov GB, Golub VO, Lvov YM, Langmuir, 18(16), 6338 (2002)
Ichinose I, Tagawa H, Mizuki S, Lvov Y, Kunitake T, Langmuir, 14(1), 187 (1998)
Ai H, Jones S, de Villiers M, Lvov Y, J. Control. Rel., 84, 59 (2003)
