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Received January 13, 2010
Accepted June 22, 2010
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Chirobiotic® T HPLC 컬럼을 이용한 Tryptophan 이성질체 분리
Separation of Tryptophan Enantiomers by using Chirobiotic® T HPLC Column
충남대학교 화학공학과, 305-764 대전시 유성구 궁동 220 1배재대학교 분자과학과, 302-735 대전시 서구 연자 1길 14
Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Korea 1Department of Molecular Science, Paichai University, 14 Yeonja 1 gil, Seo-gu, Daejeon 302-735, Korea
ihkim@cnu.ac.kr
Korean Chemical Engineering Research, August 2010, 48(4), 515-518(4), NONE Epub 8 September 2010
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Abstract
Chirobiotic® T HPLC 컬럼을 이용하여 D, L-트립토판을 분리하였다. 이동상으로 메탄올과 증류수를 각각 70/30, 80/20, 90/10의 비율로 사용하고 체류시간을 비교하기 위해 온도를 25, 40, 55 ℃로 변화시켰다. 이동상 조성비를 변화시켜 D-트립토판과 L-트립토판의 체류시간 차이를 조사하였다. 선택도와 분리도, 컬럼의 효율성을 체류시간 관점에서 계산하여 최적의 분리 조건을 찾는 실험을 하였다. 이동상에서 메탄올의 양이 감소함에 따라 트립토판 체류시간이 짧아지고, 컬럼의 온도가 증가함에 따라 체류시간도 감소한다. 메탄올과 증류수의 비가 70/30일 때와 온도가 25 ℃일 때 가장 좋은 선택도와 분리도를 얻었다.
D,L-tryptophans were separated by using Chirobiotic® T HPLC column. Mobile phases were the mixture of methanol and water(70:30, 80:20, 90:10, v/v). Experimental temperatures were adjusted as 25, 40 and 55 ℃ in order to compare retention times. Difference in D,L-tryptophan retention times was studied in terms of the interaction between stationary phase and tryptophans. Selectivity, resolution and efficiency of column were utilized to find an optimum separation condition. Retention times were shortened by increasing the amount of methanol in mobile phase and the temperature of column. The best selectivity and resolution was obtained with the temperature(25 ℃) and the ratio of mobilephase(70/30 v/v%).
Keywords
References
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Randon J, Garnier F, Rocca JL, Maisterrena B, J. Membr. Sci., 175(1), 111 (2000)
