본 연구에서는 폴리다이아세틸렌(polydiacetylene, PDA) 베시클을 이용하여 여러 가지 킬레이트제 (chelating agent)를 쉽게 검출할 수 있는 센서 시스템을 개발하였다. 다른 센서들과 비교하여 PDA기반 센서는 많은 장점이 있다. 첫째로, 형광물질의 부착이 필요 없는 무표지 검출(label-free detection)이 가능하여 실험 절차가 간단하고 빠르다. 둘째로, PDA는 청색에서 외부 자극에 의해 적색으로 변화하는 색전이를 일으키므로 육안으로 쉽게 검출을 확인할 수 있었다. 끝으로, 특정 파장에서의 colorimetric response를 측정하여 각각의 킬레이트제의 농도에 따른 정량검출도 가능하다. 본 연구에서는 5가지 종류의 킬레이트제, 즉 EDTA, EGTA, NTA, DCTA, DTPA를 PDA 베시클과 반응시켰으며, 이중에서 EDTA, DCTA는 특히 강한 반응으로 PDA의 색전이를 유도함을 알 수 있었다. 본 연구를 통하여 PDA 베시클을 사용하여 어떠한 기계나 동력을 사용하지 않고 색전이를 이용하여 킬레이트를 성공적으로 검출할 수 있음을 보여주었다.

In this research, we developed a sensor system which can easily detect several chelating agents using polydiacetylene(PDA) vesicles. In comparison to other sensors, PDA based sensor has several advantages. First, detection method is much simpler and faster because it does not require any labeling step in the experiment procedure. Second, significant color-transition from blue to red based upon external stimulus allows us the detection by naked eyes. Finally, it is also possible to perform quantitative analysis of the concentration of the chelating agent by measuring the colorimetric response. In this paper, five types of chelating agents were used, including EDTA, EGTA, NTA, DCTA and DTPA. Among them, EDTA and DCTA triggered especially strong color-transition. In conclusion, this study has led to a successful development of a color transition-based PDA sensor system for easy and rapid detection of chelating agents.

Label-Free Sensor Polydiacetylene Colorimetric Detection Chelating Agent

Lee NY, Jung YK, Park HG, Biochem. Eng. J., “On-chip Colorimetric Biosensor Based on Polydiacetylene(PDA) Embedded in Photopolymerized Poly(Ethylene glycol) Diacrylate(PEG-DA) Hydrogel", 29, 103 (2006)
Ahn DJ, Kim JM, Accounts. Chem. Res., “Fluorogenic Polydiacetylene Supramolecules: Immobilization, Micropatterning, and Application to Label-Free Chemosensors", 41, 805 (2008)
Jelinek R, Kolusheva S, Top. Curr. Chem., “Biomolecular Sensing with Colorimetric Vesicles", 277, 155 (2007)
Ji EK, Ahn DJ, Kim JM, B. Kor. Chem. Soc., “The Fluorescent Polydiacetylene Liposome", 24, 667 (2003)
Kim JM, Ji EK, Woo SM, Lee HW, Ahn DJ, Adv. Mater., “Immobilized Polydiacetylene Vesicles on Solid Substrates for Use as Chemosensors", 15(13), 1118 (2003)
Lee SS, Chae EH, Ahn DJ, Ahn KH, Yeo JK, Korea-Aust. Rheol. J., “Shear-Induced Color Transition of PDA (Polydiacetylene) Liposome in Polymeric Solutions", 19(1), 43 (2007)
Su YL, Li JR, Jiang L, Colloid. Surface. A., “A Study on the Interactions of Surfactants with Phospholipid/Polydiacetylene Vesicles in Aqueous Solutions", 257, 25 (2005)
Yoon B, Lee S, Kim JM, Chem. Soc. Rev., “Recent Conceptual and Technological Advances in Polydiacetylene-Based Supramolecular Chemosensors", 38, 1958 (2009)
Cheng Q, Stevens RC, Langmuir, “Charge-Induced Chromatic Transition of Amino Acid-Derivatized Polydiacetylene Liposomes", 14(8), 1974 (1998)
Jung YK, Park HG, Kim JM, Biosens. Bioelectron., “Polydiacetylene (PDA)-Based Colorimetric Detection of Biotin-Streptavidin Interactions", 21, 1536 (2006)
Ahn DJ, Chae EH, Lee GS, Shim HY, Chang TE, Ahn KD, Kim JM, J. Am. Chem. Soc., “Colorimetric Reversibility of Polydiacetylene Supramolecules Having Enhanced Hydrogen-Bonding under Thermal and pH Stimuli", 125(30), 8976 (2003)
Chen XQ, Lee J, Jou MJ, Kim JM, Yoon J, Chem Commun., “Colorimetric and Fluorometric Detection of Cationic Surfactants Based on Conjugated Polydiacetylene Supramolecules", 23, 3434 (2009)
Jung YK, Kim TW, Kim J, Kim JM, Park HG, Adv. Funct. Mater., “Universal Colorimetric Detection of Nucleic Acids Based on Polydiacetylene(PDA) Liposomes”, 18(5), 701 (2008)
Kim JM, Lee JS, Lee JS, Woo SY, Ahn DJ, Macromol Chem Physic., “Unique Effects of Cyclodextrins on the Formation and Colorimetric Transition of Polydiacetylene Vesicles", 206, 2299 (2005)
Lee J, Jun H, Kim J, Adv. Mater., “Polydiacetylene-Liposome Microarrays for Selective and Sensitive Mercury(II) Detection", 21(36), 3674 (2009)
Lee J, Kim HJ, Kim J, J. Am. Chem. Soc., “Polydiacetylene Liposome Arrays for Selective Potassium Detection", 130(15), 5010 (2008)
Pan JJ, Charych D, Langmuir, “Molecular Recognition and Colorimetric Detection of Cholera Toxin by Poly(Diacetylene) Liposomes Incorporating G(M1) Ganglioside", 13(6), 1365 (1997)
Rangin M, Basu A, J. Am. Chem. Soc., “Lipopolysaccharide Identification with Functionalized Polydiacetylene Liposome Sensors", 126(16), 5038 (2004)
Ryu S, Yoo I, Song S, Yoon B, Kim JM, J. Am. Chem. Soc., “A Thermoresponsive Fluorogenic Conjugated Polymer for a Temperature Sensor in Microfluidic Devices", 131(11), 3800 (2009)
Kim KW, Choi H, Lee GS, Ahn DJ, Oh MK, Colloids Surf B Biointerfaces., “Effect of Phospholipid Insertion on Arrayed Polydiacetylene Biosensors", 66, 213 (2008)
Kennedy KJ, Andras E, Elliott CM, Methven B, Can. J. Civil. Eng., “Effect of a Chelating Agent (DTPA) on Anaerobic Waste-Water Treatment in an Upflow Sludge Blanket Filter", 18, 53 (1991)
Dubrovskii IY, Tretyakov YM, Loshkarev VA, Batalina LN, Bulavko AY, Therm Eng., “Effect of Adding Chelating Agent on Behavior of Water under Supercritical Conditions in Contact with Magnetite", 25, 68 (1978)
Jung Y, Kim T, Park H, Soh H, Adv. Funct. Mater., “Specific Colorimetric Detection of Proteins Using Bidentate Aptamer Conjugated Polydiacetylene (PDA) Liposomes", 20, 3092 (2010)
Xia YT, Deng JL, Jiang L, Sensor. Actuat. B-Chem., “Simple and Highly Sensitive Detection of Hepatotoxin Microcystin-lr via Colorimetric Variation Based on Polydiacetylene Vesicles", 145, 713 (2010)
Seo D, Kim J, Adv. Funct. Mater., “Effect of the Molecular Size of Analytes on Polydiacetylene Chromism", 20(9), 1397 (2010)
Su Y, Li J, Jiang L, Colloids and Surfaces B: Biointerfaces., “Effect of Amphiphilic Molecules upon Chromatic Transitions of Polydiacetylene Vesicles in Aqueous Solutions", 39, 113 (2004)
Ahn DJ, Lee S, Kim JM, Adv. Funct. Mater., “Rational Design of Conjugated Polymer Supramolecules with Tunable Colorimetric Responses", 19(10), 1483 (2009)