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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received December 30, 2010
Accepted February 10, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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L-리보스 생산을 위한 L-아라비노스의 에피머반응

Epimerization of L-Arabinose for Producing L-Ribose

전영주 송성문 이창수 김인호^†

Young Ju Jeon Sung Moon Song Chang Soo Lee In Ho Kim^†

충남대학교 화학공학과, 305-764 대전시 유성구 궁동 220

Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejon 305-764, Korea

ihkim@cnu.ac.kr

Korean Chemical Engineering Research, October 2011, 49(5), 628-632(5), NONE Epub 30 September 2011

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Abstract

L-리보스는 항바이러스 약의 출발물질로 근래 관심의 대상이다. 이 물질은 에피머 반응에 의해 L-아라비노스로 부터 얻을 수 있다. 몰리브덴 산화물이나 몰리브덴산 촉매 그리고 메탄올/물 용액에서 에피머 반응을 수행하였다. 반응 온도, 메탄올 분율, 촉매종류를 선정하여 최적 반응을 찾고자 하였다. 이온교환크로마토그래피를 이용하여 에피머 반응물을 분리하였고 L-리보스 HPLC 크로마토그램에서 반응수율을 계산하였다. Sodex 이온교환 HPLC 칼럼과 Phenomenex NH2 HPLC 칼럼을 분석의 편의성 면에서 비교하였다. 20% 메탄올, 60 ℃, 그리고 40 g/L 몰리브덴산 조건에서 21% 최대수율을 얻었다.

L-ribose has recently attracted interest as a starting material for antiviral drug. It could be obtained from Larabinose by epimerization reaction. Epimerization reaction was carried out with molybdenium oxide or molybdic acid catalyst and methanol/water solution. Reaction temperature, methanol percentage, and catalyst kind were selected to find an optimum reaction condition. Ion exhange chromatography was used for separating epimerization reaction mixture,_x000D_ and then HPLC chromatogram of L-ribose fraction obtained to calculate the yield of the reaction. Shodex ion exchange HPLC column(Model SC1011) and Phenomenex Luna NH2 HPLC column were compared to employ a convenient HPLC analysis. It was found that the usage of 20% methanol, 60 ℃, and 40 g/L molybdic acid gives the best reaction condition with a yield of 21%.

Keywords

L-Ribose Epimerization L-Arabinose

References

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