Articles & Issues
- Language
- korean
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received January 25, 2011
Accepted February 20, 2011
- This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
SMB(Simulated Moving Bed)를 이용한 IgY(Immunoglobulin Yolk) 분리의 전산모사
Simulation of IgY(Immunoglobulin Yolk) Purification by SMB(Simulated Moving Bed)
충남대학교 화학공학과, 305-764 대전시 유성구 궁동 220
Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Korea
ihkim@cnu.ac.kr
Korean Chemical Engineering Research, December 2011, 49(6), 798-803(6), NONE Epub 28 November 2011
Download PDF
Abstract
난황에 포함된 IgY는 포유동물에 있는 바이러스나 항원에 반응하는 항체와 같은 역할을 한다. IgY 분리를 위해 난황을 전처리한 후 3-zone SMB를 이용하여 지질단백질들로 부터 분리하는 전산모사연구를 수행하였다. 회분식 크로마토그래피와 pulse input method(PIM) 실험을 이용하여 전산모사 매개변수와 흡착 등온식을 얻었다. Aspen simulator를 이용하여 전산모사를 수행하여 IgY를 분리할 수 있는 SMB 운전조건을 다음과 같이 제시할 수 있었다. 삼각형 이론의 m2와 m3 값은 각각 0.18, 1.0이고 스위칭 타임은 419 초이었다. 전산모사 결과 raffinate의 IgY 순도가 98.39%이고 두번째 싸이클에서 순도가 정상상태에 도달하였다.
IgY(Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC(High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB(Simulated Moving Bed) was adopted for continuous purification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone SMB chromatography. Before performing SMB experiments, batch chromatography and PIM(pulse input method) were performed to find operation parameters and adsorption isotherms. The results of batch chromatography were compared with simulated results using Aspen chromatography. To find the most suitable separation condition in SMB chromatography, simulations in m2-m3 plane on the triangle theory were carried out. m2 = 0.18, m3 = 1.0 and Δt = 419 s are the best_x000D_
conditions for the highest purity of IgY. With this operating parameters(flow rate in three zone and switching time), the purity of raffinate results in 98.39% from Aspen chromatography simulation. Most of the simulation reached steadystate within second recycle.
References
Akita EM, Li Chan ECY, J. Dairy Sci., 81(1), 54 (1998)
Akita EM, Nakai S, J. Food Sci., 57(3), 54 (1992)
Al-Haddad S et al., American Journal of Pathology., 155(6), 2057 (1999)
Mark CB, Robert WC, J. Catal., 25(2), 159 (1995)
Nicoud RM, Fuchs G, Adam P, Kusters E, Antia FD, Reuille F, Schmid E, Chirality., 5(4), 267 (1993)
Francotte ER, Richert P, J. Chromatogr. A., 169, 101 (1997)
Pedeferri M, Zenoni G, Mazzotti M, Morbidelli M, Chem. Eng. Sci., 54(17), 3735 (1999)
Andersson J, Mattiasson B, J. Chromatogr. A., 1107, 88 (2006)
Gottschlich N, Weidgen S, Kasche J, J. Chromatogr. A., 765(2), 201 (1997)
Chung BH, Lee CH, Lee YT, Kim IH, Korean J. Biotchnol., 14(6), 677 (1999)
Kim WK, Chung BH, Biotechnol. Bioeng., 14(3), 279 (1999)
Huntingston JA, Stein P, J. Chromatoar. B., 756, 189 (2001)
Nau F, Mallard A, Pages J, Brule G, J. Liq. Chrom.&Rel. Technol., 22(8), 1129 (1999)
Awade AC, Efstathiou T, J.Chromatoar. B., 723, 69 (1999)
Hiroko I, Noriyuki N, Toshio K, Mitsugu N, Anal. Biochem., 199, 7 (1991)
Akita EM, Nakai S, J. Food Sci., 57(3), 54 (1992)
Al-Haddad S et al., American Journal of Pathology., 155(6), 2057 (1999)
Mark CB, Robert WC, J. Catal., 25(2), 159 (1995)
Nicoud RM, Fuchs G, Adam P, Kusters E, Antia FD, Reuille F, Schmid E, Chirality., 5(4), 267 (1993)
Francotte ER, Richert P, J. Chromatogr. A., 169, 101 (1997)
Pedeferri M, Zenoni G, Mazzotti M, Morbidelli M, Chem. Eng. Sci., 54(17), 3735 (1999)
Andersson J, Mattiasson B, J. Chromatogr. A., 1107, 88 (2006)
Gottschlich N, Weidgen S, Kasche J, J. Chromatogr. A., 765(2), 201 (1997)
Chung BH, Lee CH, Lee YT, Kim IH, Korean J. Biotchnol., 14(6), 677 (1999)
Kim WK, Chung BH, Biotechnol. Bioeng., 14(3), 279 (1999)
Huntingston JA, Stein P, J. Chromatoar. B., 756, 189 (2001)
Nau F, Mallard A, Pages J, Brule G, J. Liq. Chrom.&Rel. Technol., 22(8), 1129 (1999)
Awade AC, Efstathiou T, J.Chromatoar. B., 723, 69 (1999)
Hiroko I, Noriyuki N, Toshio K, Mitsugu N, Anal. Biochem., 199, 7 (1991)