본 연구에서는 무기염인 NaCl, KCl, NaNO3 및 KNO3의 각 전해질과 L형 아미노산인 L-Valine 및 L-Proline이 용해된 아미노산/전해질 수용액에서 L-Valine 및 L-Proline의 활동도계수와 용해도를 298.15 K에서 측정하였다. 아미노산의 활동도계수는 양이온 및 음이온의 선택성 전극간의 기전력을 측정하는 전기화학 법으로 측정하였으며, 용해도는 아미노산의 고체상과 상평형을 이루고 있는 포화용액을 중량 분석하여 측정하였다. 실험적으로 측정된 전해질 및 아미노산의 활동도계수 값을 본 연구의 저자들이 수행한 지난번 연구[Korean Chem. Eng. Res. 48(4), 519(2010)]의 이론적 모델로 검토하였다. 실험을 수행한 8개의 아미노산/전해질 수용액에서 측정된 전해질 및 아미노산의 활동도계수 값은 지난번 연구의 이론적 모델에 잘 적용되는 경향을 보였으며, 또한 측정된 아미노산의 용해도 데이터도 지난번 연구_x000D_ 의 이론적 관계로 잘 묘사될 수 있었다.

Activity coefficients and solubilities of L-Valine and L-Proline in aqueous solutions containing each of four electrolytes such as NaCl, KCl, NaNO3 and KNO3 were measured at 298.15 K. The measurements of activity coefficients were carried out in the electrochemical cell coupled with two ion-selective electrodes (cation and anion), and the solubilities were measured by the gravimetric analysis of saturated solutions in equilibrium with the solid phase of amino acid. The measured activity coefficients of electrolytes and amino acids were correlated with the theoretical thermodynamic model presented in the previous work [Korean Chem. Eng. Res. 48(4), 519(2010)]. It was found that the activity coefficients of amino acids and electrolytes described based on the our previous model were well agreeable with experimental data. Also the experimental solubility data of L-Valine and L-Proline were successfully correlated with the thermodynamic relation mentioned in the previous work.

Electrolyte Amino Acid Activity Coefficient Solubility

Subramanian, Ganapathy, Bioseparations. and bioprocessing vol.1, Wiley-VCH Verlag GmbH & Co., Weinheim (2007)
Ladish MR, Bioseparations engineering : Principles, Practice and Economics, Wiley-Interscience, New York (2001)
Chen CC, Zhu Y, Evans LB, Biotechnol. Prog., 5(3), 111 (1989)
Khoshkbarchi MK, Vera JH, AIChE J., 42(8), 2354 (1996)
Khoshkbarchi MK, Vera JH, Ind. Eng. Chem. Res., 35(12), 4755 (1996)
Pazuki GR, Rohani AA, Dashtizadeh A, Fluid Phase Equilib., 231(2), 171 (2005)
Sadeghi R, Can. J. Chem., 86, 1126 (2008)
Lee BS, Kim KC, Korean Chem. Eng. Res., 48(4), 519 (2010)
Khoshkbarchi MK, Vera JH, Ind. Eng. Chem. Res., 35(8), 2735 (1996)
Chung YM, Vera JH, Fluid Phase Equilib., 203(1-2), 99 (2002)
Cohn EJ, Edsall JT, Proteins, amino acids and peptides as ions and dipolar ions, Reinhold Publishing Corp., New York (1950)
Khoshkbarchi MK, Soto-Campos, Ana M, Vera JH, J. Solution Chem., 26(10), 941 (1997)
Sotocampos AM, Khoshkbarchi MK, Vera JH, J. Chem. Thermodyn., 29(5), 609 (1997)
Soto-Campos, Ana M, Khoshkbarchi MK, Vera JH, Biophysical Chem., 67, 97 (1997)
Kamali-Ardakani M, Modarress H, Taghikhani V, Khoshkbarchi MK, J. Chem. Thermodyn., 33(7), 821 (2001)
Harris, E. L. V. and Angal, S., Protein purification methods: A practical approach, Oxford University Press, NY (1989)
Khoshkbarchi MK, Vera JH, Ind. Eng. Chem. Res., 36(6), 2445 (1997)
Lee SH, Rasaiah JC, J. Phys. Chem., 100(4), 1420 (1996)
Lu GW, Li CX, Wang WC, Wang ZH, Fluid Phase Equilib., 225(1-2), 1 (2004)
Soto A, Khoshkbarchi MK, Ver JH, Biophys. Chem., 73, 77 (1998)
Pradhan AA, Vera JH, J. Chem. Eng. Data., 45, 140 (2000)
Shuler ML, Kargi F, Bioprocess Engineering, Basic concepts, international series in the physical and chemical engineering sciences, Prentice-Hall, NY (2002)
Gross J, Sadowski G, Ind. Eng. Chem. Res., 40(4), 1244 (2001)
Chapman WG, Gubbins KE, Jackson G, Radosz M, Ind. Eng. Chem. Res., 29(8), 1709 (1990)
Huang S, Radosz M, Ind. Eng. Chem. Res., 29(11), 2284 (1990)
Lee BS, Kim KC, Korean J. Chem. Eng., 26(6), 1733 (2009)
Lee BS, Kim KC, Korean J. Chem. Eng., 27(1), 267 (2010)
Givand JC, Teja AS, Rousseau RW, AIChE J., 47(12), 2705 (2001)
Fasman GD, CRC Handbook of Biochemistry and molecular biology physical and chemical data, vol. 1, CRC press, Florida (1976)
Hamer WJ, Wu YC, J. Phys. Chem. Ref.Data., 1(4), 1047 (1972)