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Received November 17, 2011
Accepted January 6, 2012
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반응 표면 분석법을 사용한 새우껍질에서 astaxanthin 추출 조건의 최적화
Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology
인하대학교 화학공학과, 402-751 인천광역시 남구 용현동 253 1한국과학기술연구원 연료전지센터, 136-791 서울 성북구 하월곡동 39-1
Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-gu, Incheon 402-751, Korea 1Fuel Cell Research Center, Korea Institute of Science and Technology, 39-1 Hawolkok-dong, Seongbuk-gu, Seoul 136-791, Korea
rowkho@inha.ac.kr
Korean Chemical Engineering Research, June 2012, 50(3), 545-550(6), NONE Epub 5 June 2012
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Abstract
최적의 추출조건을 찾는데 매우 유용한 방법인 반응 표면 분석법(RSM, response surface methodology)을 사용하여 새우껍질로부터 astaxanthin 추출조건을 최적화하였다. 추출조건은 용매 에탄올과 추출물질의 비율, 추출온도(℃), 추출시간(min)의 세가지 독립변수를 설정하여 BBD (Box-Behnken design) 방법을 이용하였다. 이 BBD 모델링은 0.9218의 R2adj값과 0.0003의 확률 값 p 값으로 회귀 모델에 대한 신뢰도를 입증하였다. RSM 분석을 통해 찾아낸 새우껍질로부터 astaxanthin의 최적 추출조건은 에탄올 용매비 1:29.7, 추출온도 49.5 ℃, 추출시간 59.9 분이고, 이 때 astaxanthin 추출량은 17.80 μg/g으로 예측하였다. 최적 수율로 예측된 결과는 각각의 조건에 따른 실험을 통해 그 예측의 정확도를 확인하였으며 17.77 μg/g으로 예측조건과 비슷한 결과를 보였다.
A 17-run Box-Behnken design (BBD) was used to optimize the extraction conditions of astaxanthin from shrimp waste. Three factors such as ratio of ethanol to raw material, extraction temperature (℃) and extraction time (min) were investigated. The adjusted coefficient of determination (R2adj) for the model was 0.9218, and the probability value (p=0.0003) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized ratio of ethanol to raw material 29.7, extraction temperature 49.5 ℃ and extraction time 59.9 min. Under these conditions, the mean extraction yield of astaxanthin was 17.80 μg/g, which was in good agreement with the predicted model value. Under these conditions, validation experiments were done and the mean extraction yield of astaxanthin was 17.77 μg/g, which is in good agreement with the predicted model value.
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