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Received May 22, 2013
Accepted June 30, 2013
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오매 추출물에 함유된 Ursolic Acid에 의한 Helicobacter pylori의 Urease 활성억제
Ursolic Acid Isolated from Mume Fructus Inhibits Urease Activity of Helicobacter pylori
다림바이오텍, 445-746 경기 화성시 향남읍 상신리 907-5 1경희대학교 화학공학과, 446-701 경기 용인시 기흥구 서천동 1
Central Research Center, Dalim Biotech, 907-5 Sangsin-ri, Hyangnam-eup, Hwasung-si, Gyeonggi-do 445-746, Korea 1Department of Chemical Engineering, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Korea
chpark@khu.ac.kr
Korean Chemical Engineering Research, October 2013, 51(5), 591-596(6), 10.9713/kcer.2013.51.5.591 Epub 1 October 2013
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Abstract
전통식물한약재에 대한 데이터베이스, 한국식물자원에 대한 문헌 및 선행연구 결과에 근거해 선정한 6가지의 식물 한약재(연교, 소목, 형개, 황련, 소엽, 오매)의 에탄올(70%) 추출물 중에서 오매 추출물이 Helicobacter pylori의 urease 활성에 가장 강한 억제력을 나타내었다. 극성이 다른 용매를 사용한 회분식 용매추출과 칼럼 크로마토그래피를 이용한 3단계 분리공정을 통하여 오매 추출물에 함유된 유효성분을 분리·정제하였다. NMR 분석에 의해 정제된 오매 추출물에 함유된 활성성분이 ursolic acid로 확인되었다. 본 실험결과는 오매에 함유된 이 성분이 항생제를 대신하여 H. pylori 제거에 활용될 수 있음을 시사하였다.
Urease activity of Helicobacter pylori was most strongly inhibited by extract of Mume Fructus among ethanol (70%, v/v) extract of 6 herbal materials selected from our previous work, database on traditional herbal materials, and literature data on Korean plant resources. Active compounds in the extract of Mume Fructus were separated by batch extraction, and further purified by chromatography in a silica gel column and an octadecyl silica gel column using solvents of different polarity. By NMR analysis of the last chromatographic fraction we identified ursolic acid as the active compound of urease inhibition. The result suggests that this component in Mume Fructus can possibly be used for the eradication of H. pylori.
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Kubo J, Lee JR, Kubo I, Agric. Food Chem., 47, 533 (1999)
Yee YK, Koo MWL, Szeto ML, J. Gastroenterol.Hepatol., 17, 552 (2002)
Yoon YS, Lee SH, Baek NI, Kim HY, Park CH, Korean J. Biotechnol. Bioeng., 19, 187 (2004)
Shin SJ, Park CE, Baek NI, Chung IS, Park CH, Biotech.Bioproc. Eng., 14, 140 (2009)
Shi DH, Liu YW, Liu WW, Gu ZF, Pharm Biol., 49, 752 (2011)
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