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Received August 12, 2013
Accepted November 7, 2013
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향상된 가수분해율을 얻기 위한 전자선 조사와 물찜의 복합 전처리공정을 이용한 케냐프 코어 전처리
Pretreatment of Kenaf Core by Combined Electron Beam Irradiation and Water Steam for Enhanced Hydrolysis
한국원자력연구원 공업환경연구부, 580-185 전북 정읍시 금구길 29
Research Division for Industry & Environment, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeonbuk 580-185, Korea
jpjeun@kaeri.re.kr
Korean Chemical Engineering Research, February 2014, 52(1), 113-118(6), 10.9713/kcer.2014.52.1.113 Epub 3 February 2014
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Abstract
케냐프 코어 전처리를 위하여 전자선 조사와 물찜이 결합된 복합 전처리공정에 대해 연구하였다. 각 시료는 50에서 1,000 kGy까지의 선량으로 조사한 후, 오토클레이브를 이용하여 120 ℃에서 5시간 동안 물찜처리를 하였다. 적외선 분광기와 X선 회절 분석기를 사용하여 전처리 되지 않은 시료와 전처리된 시료의 분자 구조와 결정도 변화를 분석하였다. 전처리 되지 않은 시료의 결정화도 지수는 50.6%에서 500 kGy 조사된 시료는 55.0%로 증가함을 알 수 있었다. 그 다음, 전처리된 시료에 비활성도 70 FPU/mL, 40 CBU/mL의 효소를 주입하여 생산된 단당류 총합으로 당화율을 구하였다. 이 때 효소 가수분해 시간은 24, 48, 72시간으로 하였다. 500 kGy로 조사된 시료의 72 시간 가수분해 후 당화율은 83.9%로 가장 높게 나타났고, 전처리된 시료의 당화율은 조사량 증가에 따라 100 kGy에서 50.8%, 200 kGy에서 58.6%, 300 kGy에서 67.9%로 각각 증가하였다.
We have investigated the combined pretreatment of electron beam irradiation (EBI) and water steam as a kenaf core pretreatment process. After each sample was exposed to electron beam dose ranging from 50 to 1,000 kGy, the irradiated sample was treated by water steam using an autoclave for 5-h at 120 ℃. The pretreated samples were characterized using FTIR-ATR and XRD. FTIR spectra and XRD analysis of nonpretreated and pretreated samples confirm that crystallinity changes were observed before and after the pretreatment. The crystallinity index (CrI) was increased from 50.6% for nonpretreated sample 55.0% for 500 kGy exposed sample. And then, we analyzed sugar yield that is the amount of produced mono-saccharides in pretreated sample by enzymatic hydrolysis; an enzyme activity rate was 70 FPU/mL and 40 CBU/mL, and the loading time was 24, 48 and 72-h. The highest sugar yield was 83.9% at 500 kGy after 72-h for enzymatic hydrolysis. The sugar yield of enzymatic hydrolysis for pretreatment samples was increased as doses are subsequently changed to 100, 200 and 300 kGy, allowing to give 50.8%, 58.6% and 67.9%, respectively.
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Ebringerova A, Hromadkova Z, Heinze T, Adv. Polym. Sci., 186, 1 (2005)
Sanchez OJ, Cardona CA, Bioresour. Technol., 99(13), 5270 (2008)
Zhu JY, Pan XJ, Wang GS, Gleisner R, Bioresour. Technol., 100(8), 2411 (2009)
Zheng Y, Pan Z, Zhang R, Int. J. Agric. Biol. Eng., 2, 51 (2009)
Guo B, Zhang Y, Ha SJ, Jin YS, Morgenroth E, Bioresour. Technol., 110, 278 (2012)
Carter B, Squillace P, Gilcrease PC, Menkhaus TJ, Biotechnol. Bioeng., 108(9), 2053 (2011)
Limayem A, Ricke SC, Prog. Energy Combust. Sci., 38, 449 (2012)
Binod P, Sindhu R, Singhania RR, Vikram S, Devi L, Nagalakshmi S, Kurien N, Sukumaran RK, Pandey A, Bioresour. Technol., 101(13), 4767 (2010)
Menon V, Rao M, Prog. Energy Combust. Sci., 38, 522 (2012)
Kim TH, Korean J. Chem. Eng., 28(11), 2156 (2011)
Khan AW, Biotechnol. Bioeng., 28, 1449 (1986)
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Driscoll M, Stipanovic A, Winter W, Kun C, Manning M, Jesica S, Radiat. Phys. Chem., 78, 539 (2009)
Alvira P, Tomas-Pejo E, Ballesteros M, Negro MJ, Bioresour. Technol., 101(13), 4851 (2010)
Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M, Bioresour. Technol., 96(6), 673 (2005)
Liu, S. J., Biotechnol. Adv., 28, 563 (2010)
Shin SJ, Sung YJ, Radiat. Phys. Chem., 77, 1034 (2008)
Chosdu R, Hilmy N, Erizal Erlinda TB, Abbas B, Radiat. Phys. Chem., 42, 695 (1993)
Ozturk I, Irmak S, Hesenov A, Erbatur O, Biomass Bioenerg., 34(11), 1578 (2010)
Badal CS, Tsuyoshi Y, Michael AC, Kenji S, Ind. Crop. Prod, 44, 367 (2013)
Lawther JM, Sun R, Banks WB, Holzforschung, 50, 365 (1996)
Selig M, Weiss N, Ji Y, “Enzymatic Saccharification of Lignocellulosic Biomass: Laboratory Analytical Procedure(LAP),” National Renewable Energy Laboratory, Golden, CO, USA (2008)
Kim JS, Korean Chem. Eng. Res., 51(3), 303 (2013)
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D, “Determination of Structural Carbohydrates and Lignin in Biomass: Laboratory Analytical Procedure (LAP),” National Renewable Energy Laboratory, Golden, CO, USA (2008)
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Okuda N, Hori K, Sato M, J. Wood Sci., 52, 249 (2006)
Kumar P, Barrett DM, Delwiche MJ, Stroeve P, Ind. Eng. Chem. Res., 48(8), 3713 (2009)
Hu F, Ragauskas A, Bioenerg. Res., 5, 1043 (2012)
Kumar R, Mago G, Balan V, Wyman CE, Bioresour. Technol., 100(17), 3948 (2009)
Xiao B, Sun XF, Sun RC, Polym. Degrad. Stabil., 74, 307 (2001)
Hsu TC, Guo GL, Chen WH, Hwang WS, Bioresour. Technol., 101(13), 4907 (2010)
Chundawat PSS, Venkatesh B, Dale BE, Appl. Biochem. Biotechnol, 96, 219 (2006)
Chabannes M, Plant J., 28, 271 (2001)
Minu K, Jiby KK, Kishore VVN, Biomass Bioenerg., 39, 210 (2012)
Zhao XB, Wang L, Liu DH, J. Chem. Technol. Biotechnol., 83(6), 950 (2008)
Kumakura M, Kaetsu I, Radiat. Phys. Chem., 23, 523 (1984)
Gumuskaya E, Usta M, Krici H, Polym. Degrad. Stabil., 81, 559 (2003)
Chen WH, Ye SC, Sheen HK, Appl. Energ., 93, 237 (2012)
Saha BC, Yoshidaa T, Cotta MA, Sonomoto K, Ind. Crop. Prod., 44, 367 (2013)
Karthika K, Arun AB, Rekha PD, Carbohydr. Polym., 90, 1038 (2012)
Wang W, Yuan T, Wang K, Cui B, Dai Y, Bioresour. Technol., 107, 282 (2012)
