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Received June 24, 2014
Accepted August 17, 2014
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초임계 이산화탄소를 이용한 Nannochloropsis sp. 미세조류로부터 바이오디젤 생산용 지질의 추출
Lipid Extraction from Nannochloropsis sp. Microalgae for Biodiesel Production Using Supercritical Carbon Dioxide
(주)한울엔지니어링 기술연구소, 435-733 경기도 군포시 고산로148번길 17 1한양대학교 화학공학과, 426-791 경기도 안산시 상록구 한양대학로 55 2공주대학교 환경공학과, 331-717 충청남도 천안시 서북구 천안대로 1223-24
R&D Center, Hanwoul Engineering Co., Ltd., 17 Gosan-ro 148beon-gil, Gunpo-si, Gyeonggi 435-733, Korea 1Department of Chemical Engineering, Hanyang University, 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi 426-791, Korea 2Department of Environmental Engineering, Kongju National University, 1223-24 Cheonan-daero, Seobuk-gu, Cheonan-si, Chungnam 331-717, Korea
kh@hanwoul.com
Korean Chemical Engineering Research, April 2015, 53(2), 205-210(6), 10.9713/kcer.2015.53.2.205 Epub 30 March 2015
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Abstract
본 연구에서는 미세조류인 Nannochloropsis sp.로부터 바이오디젤 생산용 지질을 얻기 위하여 유기용매 및 초임계 이산화탄소(SC-CO2)를 이용하여 추출을 수행하였다. SC-CO2 추출법으로 얻은 지질의 지방산메틸에스테르 함량은 58.31%로 높았으며, Bligh-Dyer 추출법은 18.0 wt.%의 가장 높은 조지방 수율을 나타내었다. SC-CO2 추출법에 극성을 높이기 위해 공용매로서 methanol을 사용한 결과, 조지방 수율 12.5 wt.%, 지방산메틸에스테르 함량 56.32%, 지방산메틸에스테르 수율 7.04 wt.%였으며, SC-CO2 만을 이용하는 추출 방법에 비하여 추출 시간을 2시간에서 30분으로 단축시킬 수 있었다. 따라서 미세조류에서 지질을 추출하는데 기존의 유기용매 추출법과 비교하여 SC-CO2 추출법이 친환경적이며, 효율적인 방법임을 확인하였다.
In this paper, microalgae lipid extractions were performed using conventional organic solvent and supercritical carbon dioxide (SC-CO2) for biodiesel-convertible lipid fractions. The highest levels (58.31%) of fatty acid methyl ester (FAME) content in the lipid extracted by SC-CO2 was obtained, and 18.0 wt.% crude lipid yield was achieved for Bligh-Dyer method. In the SC-CO2 extraction, methanol as a co-solvent was applied to increase the polarity of extract. The experimental results indicated that crude lipid yield, FAME content and yield extracted by combination of SC-CO2 with methanol were 12.5 wt.%, 56.32% and 7.04 wt.%, respectively, and this method could reduce the extraction time from 2 hour to 30 min when compared to SC-CO2 extraction. Therefore, SC-CO2 extraction is proven to be an environmentally-friendly and an effective method for lipid extraction from microalgae.
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