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Received July 3, 2014
Accepted October 1, 2014
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탈지미강의 아임계수 가수분해 생성물을 배지로 이용한 Saccharomyces cerevisiae의 배양
Cultivation of Saccharomyces cerevisiae using Defatted Rice Bran Hydrolyzed in Near-critical Water as a Culture Medium
한국과학기술연구원 청정에너지연구센터, 136-791 서울 성북구 화랑로14길 5 1서울대학교 화학생물공학부, 151-742 서울 관악구 관악로 1
Clean Energy Research Center, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul 136-791, Korea 1School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea
Korean Chemical Engineering Research, April 2015, 53(2), 211-215(5), 10.9713/kcer.2015.53.2.216 Epub 30 March 2015
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Abstract
아임계수를 이용하여 탈지미강의 가수분해를 실시하고, 결과로 얻어진 가수분해물이 배지로써 이용할 수 있는지 검토하기 위해 Saccharomyces cerevisiae의 배양을 시도해보았다. 아임계수 가수분해 반응은 회분식 반응을 통해 이루어졌으며, 생성물에 포함된 전당, 이당류, 단당류의 함량, 총유기탄소 (TOC), 총질소 (TN), pH를 측정하였다. Saccharomyces cerevisiae의 성장 속도는 탁도 측정을 통해 확인하였다. 전당 수율, TOC, TN은 240 oC까지 온도에 따라 증가하다가 그 이상의 온도에서는 감소하였다. 240 oC 이상에서의 수율 감소는 유기산 생성에 기인한 것이고 이는 pH 변화를 통해 확인할 수 있었다. 포도당의 수율은 200 oC에서 최대였고 그 이상의 온도에서는 빠르게 감소하였다. 탈지미강 가수분해물에서 배양된 Saccharomyces cerevisiae의 성장 속도는 일부 조건에서 상용 배지와 근접한 수준을 보였고, 성장 속도는 포도당의 함량과 상관 관계가 있었다.
The hydrolysis of defatted rice bran using near-critical water was performed, and the feasibility of consequent hydrolyzate as a growth medium was investigated by the cultivation of Saccharomyces cerevisiae. The near-critical water hydrolysis was carried out through a series of batch experiments, and the contents of total carbohydrates, disaccharides, and monosaccharides, total organic carbon (TOC), total nitrogen (TN), pH of products were measured. The growth rate of Saccharomyces cerevisiae was measured with optical density. The yield of total carbohydrates, TOC, and TN increased with temperature below 240 oC, however, decreased above 240 oC. The decrease of yields above 240 oC was caused by the formation of organic acids, and it agreed with the change of pH of products. The yield of glucose was a maximum at 200 oC and it decreased dramatically at higher temperature. The growth rate of Saccharomyces cerevisiae cultivated in the hydrolyzate was similar with that in the commercial medium under certain conditions. The growth rate was correlated with the content of glucose in hydrolyzate.
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Saha BC, Cotta MA, Biotechnol. Prog., 22(2), 449 (2006)
Kim TH, Korean J. Chem. Eng., 28(11), 2156 (2011)
Kim KS, Kim JS, Korean Chem. Eng. Res., 50(1), 18 (2012)
Toor SS, Rosendahl L, Rudolf A, Energy, 36(5), 2328 (2011)
Brunner G, J. Supercrit. Fluids, 47(3), 373 (2009)
Pettersen RC, Adv. Chem., 207, 57 (1984)
