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Received September 22, 2014
Accepted October 26, 2014
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초임계유체를 이용한 고삼으로부터 Genistein의 추출
Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide
전남대학교 응용화학공학부, 500-757 광주시 북구 용봉동 300번지
Department of Applied Chemical Engineering, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Korea
chkang@chonnam.ac.kr
Korean Chemical Engineering Research, August 2015, 53(4), 445-449(5), 10.9713/kcer.2015.53.4.445 Epub 29 July 2015
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Abstract
본 연구에서는 초임계 이산화탄소를 용매로 사용하여 고삼으로부터 genistein을 추출하였다. 공용매와 추출 온도 및 압력 등에 따른 추출효율을 측정하였으며, 공용매로 메탄올과 에탄올을 사용하여 200 bar에서 300 bar의 압력범위와 35 oC와 50 oC의 온도범위에서 연구를 수행하였다. 조건에 따라 서로 다른 genistein의 추출효율을 보였으며, 특히 높은 압력과 높은 온도에서 가장 높은 추출효율이 관찰되었다. 또한 메탄올이 에탄올보다 더욱 효과적인 공용매 특성을 보였으며 농도가 높을수록 추출효율도 증가하였다. DPPH 라디칼 소거 활성을 측정하여 고삼 추출물의 항산화 활성을 비교하였다.
This study was directed to finding an optimum extraction condition of genistein from the S. flavescens with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, temperature and a co-solvent on the extraction efficiency were investigated. The aqueous ethanol and methanol solutions were used as co-solvents while the tested operating pressure and temperature ranges were from 200 bar to 300 bar and from 308.15 K to 323.15 K, respectively. The concentration of genistein was determined by means of HPLC equipped with a UV detector. From the results, it was observed that an increase in pressure led to the higher extraction efficiency. Further, methanol showed better performance as a co-slovent than ethanol. The DPPH radical scavenging activities were measured to compare antioxidant activities of S. flavescens extracts.
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