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Received March 13, 2015
Accepted May 4, 2015
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알루미늄 표면에 생물막의 형성인자 및 항균제에 의한 생물막 제거효과 분석
Study of Formation Factor of Biofilm on Aluminum surface and Removal Efficiency of Biofilm by Antimicrobials
서울시립대학교 화학공학과, 02792 서울특별시 동대문구 시립대로 163 1FITI시험연구원 환경바이오평가팀, 28115 충청북도 청주시 청원구 오창읍 양청3길 21
Department of Chemical Engineering, University of Seoul, 163 Siripdae-ro, Dongdaemun-gu, Seoul 02792, Korea 1Environment & Assessment Team, FITI Testing & Research Institute, 21, Yangcheong 3-gil, Ochang-eup, Cheongwon-gu, Cheongju si, Chungcheongbuk-do 28115, Korea
sjpark@fiti.re.kr
Korean Chemical Engineering Research, December 2015, 53(6), 730-739(10), 10.9713/kcer.2015.53.6.730 Epub 30 November 2015
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Abstract
에어컨을 작동 시 악취가 심하게 느껴지는 차량 25대에서 총 108종의 미생물(곰팡이 79종, 세균 29종)이 분리되었으며, 곰팡이 43종, 세균 23종이 동정되었다. 차량 내 에어컨 작동 시 발생하는 응축수를 분석한 결과 pH 6.4~7.1, 탄소원 12.5~34.2 mg/L, 질소원 0.9~18.6 mg/L, 이온성분 0.5~27.8 mg/L, 미네랄성분 0.1~7.7 mg/L로 분석되었다. 생물막은 중온성 세균에 의해 여름철 온·습도 조건(26 oC, 70% R.H.)에서 형성되었고, 생물막을 인위적으로 제거하더라도 환경인자(영양성분 및 온·습도)가 적합하면 재생됨이 확인되었다. 항균제의 항균 활성은 부유성 세균에 대해 15분 이내에 99.9%의 균 감소율을 보였지만, 항균제의 생물막 제거효과는 없었다. 항균제를 처리한 차량 내에서는 에탄올 성분이 최대 1,940 ppmv이 검출되었고, 에탄올의 흡입 시 급성독성치 보다는 작았지만, 사용된 화학물질들의 인체 안전성에 대한 검토가 필요하다.
108 microorganism types (79 types of fungi and 29 types of bacteria) were isolated from 25 automobiles generating bad odor when the air conditioner was turned on, and 43 types of fungi and 23 types of bacteria were identified. The analysis of condensate generated by the air conditioners in the automobiles indicated pH 6.4~7.1, 12.5~34.2 mg/L carbon source, 0.9~18.6 mg/L nitrogen source, 0.5~27.8 mg/L ion contents, and 0.1~7.7 mg/L mineral contents. The biofilms were formed by the mesophiles under the summer temperature/humidity condition (26 oC, 70% R.H.), and they were regenerated when the environmental factors (nutritional contents and temperature/humidity) were appropriate even after they were artificially removed. Although the antimicrobials removed 99.9% of planktonic cells within 15 minutes, they were not effective in removing biofilm. Up to 1,950 ppmv of ethanol was observed in the automobile treated with the antimicrobials. Although the figure is lower than the acute toxicity level when inhaled by humans, the health safety of the chemical substances used in the antimicrobials needs to be reviewed.
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