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Received February 5, 2015
Accepted May 19, 2015
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창자파래로부터 citrate buffer를 이용한 전처리와 효소가수분해를 통한 환원당 생산
Production of Total Reducing Sugar from Enteromorpha intestinalis Using Citrate Buffer Pretreatment and Subsequent Enzymatic Hydrolysis
부경대학교 생물공학과, 48513 부산광역시 남구 용소로 45 1전남대학교 생물공학과, 61186 광주광역시 북구 용봉로 77
Department of Biotechnology, Pukyong National University, 45, Yongso-ro, Nam-gu, Busan, 48513, Korea 1Department of Biotechnology and Bioengineering, Chonnam National University, 77, Yongbong-ro, Buk-gu, Gwangju, 61186, Korea
gtjeong@pknu.ac.kr
Korean Chemical Engineering Research, February 2016, 54(1), 70-74(5), 10.9713/kcer.2015.54.1.70 Epub 12 February 2016
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Abstract
본 연구에서는 창자파래(Enteromorpha intestinalis)로부터 citrate buffer를 사용하여 전처리 조건(고액비, 반응온도, buffer의 pH와 농도)에 따른 전처리 반응과 효소가수분해를 통한 가수분해 수율을 조사하였다. 0.25 M, pH 3.5의 citrate buffer를 이용하여 140 oC에서 60분간 전처리를 수행한 결과, 5.40%의 가수분해 수율을 얻었다. 최종적으로 전처리 반응 후 24시간의 효소 가수분해를 통하여 18.68%의 가수분해 수율을 얻었다. 이 결과는 대조구에 비하여 약 1.81배 증가한 결과이다.
In this study, the effects of citrate buffer pretreatment conditions (solid-to-liquid ratio, reaction temperature, pH and concentration of buffer) on enzymatic hydrolysis of E. intestinalis for total reducing sugar (TRS) production were investigated. As a results of the citrate buffer pretreatment, a 5.40% hydrolysis yield was obtained under conditions including 1:10 solid-to-liquid ratio, 0.25 M citrate buffer (pH 3.5) at 140 oC for 60 min. The maximum hydrolysis yield of 18.68% was obtained to enzymatic hydrolysis after pretreatment. This result is 1.81 times higher than that of control.
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References
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Jeong GT, Park DH, Appl. Biochem. Biotechnol., 161(1-8), 41 (2010)
Jang SS, Shirai Y, Uchida M, Wakisaka M, African J. Biotechnol., 11(8), 1953 (2012)
Cho YK, Kim MJ, Kim SK, KSBB J., 28(6), 366 (2013)
Jang JS, Cho Y, Jeong GT, Kim SK, Bioprocess. Biosyst. Eng., 35, 11 (2012)
Kim DH, Lee SB, Jeong GT, Bioresour. Technol., 161, 348 (2014)
Feng D, Liu H, Li F, Jiang P, Qin S, Chin J. Oceanol. Limnol., 6, 1243 (2011)
Sanchez-Machado DI, Lopez-Cervantes J, Paseiro-Losada P, Lopez-Hemandez J, Food Chem., 85, 439 (2004)
Kim AR, Kim DH, Jeong GT, KSBB J., http://dx.doi.org/10.7841/ksbbj.2015.30.2.1, 30 (2015)
Miller GL, Anal. Chem., 31, 426 (1959)
Park DH, Jeong GT, Korean Chem. Eng. Res., 51(1), 106 (2013)
Meinita MDN, Hong YK, Jeong GT, Bioprocess. Biosyst. Eng., 35, 123 (2012)
