리모넨은 오렌지 향이 있는 천연의 키랄 화합물로 주로 감귤껍질과 레몬껍질에 함유되어 있다. 4 oC로 냉장 보관한 감귤 껍질을 에탄올을 용매로 속슬렛 추출기에서 2시간동안 120 oC에서 추출하였다. 역상 HPLC 분석을 통해 d-리모넨과 불순물의 헨리 상수를 계산하여 HLim=8.55, Himp=0.223를 얻었다. Aspen chromatography 프로그램을 사용해서 0.46×25 cm 칼럼으로 이루어진 4-bed SMB의 리모넨 전산모사를 수행하였고 삼각도내의 m2, m3 값을 변경하면서 순도가 가장 높은 분리 조건을 찾았다. 그 결과 가장 높은 순도는 98.59%이고, m2=2.57, m3=9.55였다. 이 때의 feed 유량은 1 mL/min, desorbent 유량은 1.19 mL/min, extract 유량은 0.857 mL/min, raffinate 유량은 1.34 mL/min이었다. Scale-up 전산모사를 위해 칼럼의 직경을 1.6 cm로 늘린 4-bed SMB에서 직경이 0.46 cm인 4-bed SMB와 같은 결과를 갖는 조건을 찾기 위해 유량을 칼럼 부피 비에 정비례하여 증가시켰다. 이 때 feed, desorbent, extract, raffinate의 유량은 각각 12 mL/min, 14 mL/min, 10 mL/min, 16 mL/min이었다. 리모넨과 불순물의 등온흡착곡선을 선형으로 가정하였기에 칼럼 부피에 정비례하여 유량을 증가시키는 scale-up이 가능하였다.

Limonene is orange flavored natural material that is mainly contained in mandarine and lemon peels. Dlimonene was extracted from cold-storaged mandarine peel by using Soxhlet extractor at 120 oC for 2 hours with ethanol as solvent. Henry constants of d-limonene and impurity were calculated as HLim=8.55 and Himp=0.223 from the result of HPLC analysis. 4-bed SMB of limonene simulation with 0.46×25 cm columns was conducted by using Aspen chromatography program. Then effective condition for purity was found by changing m2 and m3 values in triangle diagram. The highest purity was 98.59% at m2=2.57, m2=9.55. For this case, feed, desorbent, extract, and raffinate flow rates were 1 mL/min, 1.19 mL/min, 0.857 mL/min and 1.34 mL/min, respectively. Scale-up simulation was also conducted by increasing column diameter from 0.46 cm to 1.6 cm for getting the same efficiency. The increased flow rates were 12 mL/min, 14 mL/min, 10 mL/min, and 16 mL/min for feed, desorbent, extract, and raffinate. It was possible to scale-up with maintaining same limonene purity because linear isotherms of limonene and impurity were assumed.

Simulated Moving Bed (SMB) d-limonene Aspen Chromatography

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