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Language: korean

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 21, 2015
Accepted September 8, 2015

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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식물세포배양으로부터 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리 양상

Separation Behavior of Paclitaxel and Its Semi-synthetic Precursor 10-Deacetylpaclitaxel from Plant Cell Cultures

이충기 김진현^†

Chung-Gi Lee Jin-Hyun Kim^†

공주대학교 화학공학부, 31080 충남 천안시 서북구 천안대로 1223-24

Department of Chemical Engineering, Kongju National University, 1223-24, Cheonan-daero, Seobuk-gu, Cheonan, Chungnam, 31080, Korea

Korean Chemical Engineering Research, February 2016, 54(1), 89-93(5), 10.9713/kcer.2016.54.1.89 Epub 12 February 2016

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Abstract

본 연구에서는 식물세포배양으로부터 항암물질 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리양상을 조사하였다. 식물세포인 바이오매스를 이용한 분리/정제 공정인 용매를 이용한 바이오매스 추출, 액-액 추출, 흡착제 처리, 헥산 침전, 분별 침전을 순차적으로 수행한 결과, 흡착제 처리 공정에서 10-디아세틸파클리탁셀는 파클리탁셀로부터 가장 효과적으로 분리됨을 알 수 있었다. 파클리탁셀 및 10-디아세틸파클리탁셀 분리에 적합한 흡착제 종류, 건조시료/흡착제 비율, 흡착제 처리 온도는 각각 실로퓨트, 1:1.5(w/w), 20 oC 이었다. 최적의 흡착제 처리 조건에서 10-디아세틸파클리탁셀은 74.1% 분리/회수 가능하였다.

In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and 20 oC, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.

Keywords

Paclitaxel 10-Deacetylpaclitaxel Separation Adsorbent Treatment Optimization

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