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- In relation to this article, we declare that there is no conflict of interest.
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Received July 18, 2015
Accepted October 1, 2015
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미세접촉인쇄법을 이용한 지방세포 칩 제작
Simple Fabrication of Adipocyte Cell Chip Using Micropatterning
1Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 29, Geumgu-gil, Jeongeup, Jeonbuk, 56212, Korea 2Department of Chemical Engineering, Chungnam National University, 220, Gung-Dong, Yuseong-gu, Daejeon, 34134, Korea 3, Korea
rhadum@cnu.ac.kr
Korean Chemical Engineering Research, April 2016, 54(2), 223-228(6), 10.9713/kcer.2016.54.2.223 Epub 5 April 2016
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Abstract
본 논문은 PDMS을 기반으로 한 미세접촉인쇄법을 사용하여 보다 효율적으로 지방세포를 선택적으로 배양접시에 부착시켰으며 패터닝 된 배양접시에서 지방세포가 빠르게 지방분화를 가능하게 하였다. PDMS을 이용한 선택적 표면처리 방법은 기존의 세포칩 제작과 달리 소량의 유기용매사용, 저가의 장비 사용, 또한 효율적으로 특정 지역 안에서 지방세포를 고정화하고 성장시킬 수 있으며 이를 통해 지방세포의 생리학적 상태를 손쉽게 관찰할 수 있는 분석 기술로 활용된다. 이렇게 구성된 지방세포칩 위에 대표적인 비만 억제제 약물인 Orlistat을 0.2 μM~5.0 μM 농도로 처리하였을 때 대조군에 비하여 최대 26.5%의 억제 효과가 나타남을 확인하였다. 따라서, 본 논문에서 제안하는 방법을 통하여 다양한 비만 억제제 약물 탐색을 위한 세포칩으로 활용이 가능할 것으로 기대된다.
In this study, we described a simple and facile method to generate uniform microwells poly(dimethyl siloxane) (PDMS) microstamps through micro-molding for efficient, rapid and reliable cell patterning of adipocyte differentiation. In contrast to the conventional methods, the microstamp technologies are low expensive, non-toxic, and using a small amount of solution. Recently, Orlistat known as tetrahydrolipstatin is a prescription drug designed to treat obesity which is used to aid in weight loss and help to reduce overweight obesity. Here, 3T3-L1 cells were treated under various concentration manners of Orlistat 0.2 μM~5.0 μM. and it was confirmed maximum 26.5% inhibition activity compared to control. Thus, we elucidated this platform can be used for the real-time analyzing of cell proliferation, adipocyte differentiation for evaluation of anti-obesity agents on cell chip. Furthermore, we except that this platform technology designed here might be readily be expanded to discover a wider variety of anti-obesity agents.
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